Palmitoleic acid has stronger anti-inflammatory potential in human endothelial cells compared to oleic and palmitic acids
| Autor: | Camila Oliveira de Souza, Carina A. Valenzuela, Philip C. Calder, José Cesar Rosa Neto, Elizabeth A. Miles, Ella J. Baker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system Cell Survival Peroxisome proliferator-activated receptor Palmitic Acids 030204 cardiovascular system & hematology Cell Line Fatty Acids Monounsaturated Palmitic acid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Human Umbilical Vein Endothelial Cells medicine Humans Palmitoleic acid Chemokine CCL5 Chemokine CCL2 Inflammation chemistry.chemical_classification Interleukin-6 Tumor Necrosis Factor-alpha Monocyte Anti-Inflammatory Agents Non-Steroidal Endothelial Cells Intercellular Adhesion Molecule-1 Endothelial stem cell Oleic acid 030104 developmental biology Endocrinology medicine.anatomical_structure Gene Expression Regulation chemistry Arachidonic acid Tumor necrosis factor alpha Oleic Acid Food Science Biotechnology |
| Popis: | 1 ScopeFatty acids (FAs) may affect endothelial cell (EC) function, influencing atherogenesis and inflammatory processes. Palmitoleic acid (POA) has been described as an anti‐inflammatory FA. However, its effects on ECs are underexplored. This study compares the effects of POA with those of palmitic acid (PA) and oleic acid (OA) on EC inflammatory responses.2 Methods and ResultsEAHy926 cells (EC lineage) are exposed to PA, OA, or POA, and stimulated with tumor necrosis factor (TNF)‐α. Associated with the FA's own incorporation, PA induces a twofold increase in arachidonic acid, while POA increases the amount of cis‐vaccenic acid. PA, but not OA, enhances the production of IL‐6 and IL‐8 in response to TNF‐α. In contrast, POA decreases production of monocyte chemotactic protein (MCP)‐1, IL‐6, and IL‐8 compared to PA. TNF‐α increases surface intercellular adhesion molecule‐1 expression previously decreased by POA. TNF‐α stimulation increases the expression of NFκB, cyclooxygenase (COX)‐2, MCP‐1, and IL‐6 genes and reduces the expression of peroxisome proliferator‐activated receptor (PPAR)‐α gene. PA enhances the expression of MCP‐1, IL‐6, and COX‐2 genes, while POA downregulates these genes, decreases expression of NFκB, and upregulates PPAR‐α gene expression.3 ConclusionPOA has anti‐inflammatory effects on ECs stimulated with TNF‐α and may counter endothelial dysfunction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|