Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities
| Autor: | Athena Yerganian, Robert N. O'Meally, Michael A. Bowen, Venkata Gayatri Dhara, Kelley M. Heffner, Swetha Kumar, Natalia I. Majewska, Robert Cole, George Yerganian, Michael J. Betenbaugh, Deniz Baycin Hizal, Diane Hatton, Jie Zhu |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DNA Replication Proteomics Quantitative proteomics Hamster lcsh:Medicine CHO Cells Biologics Kidney Proteome informatics Chinese hamster Article 03 medical and health sciences Cricetulus Tandem Mass Spectrometry Animals lcsh:Science Lung Multidisciplinary 030102 biochemistry & molecular biology biology Chemistry Chinese hamster ovary cell Myocardium lcsh:R Cell Cycle Ovary Brain Proteins Phospholipid transport biology.organism_classification Chromosomes Mammalian Cell biology 030104 developmental biology Cell culture Proteome lcsh:Q Female Spleen |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Chinese hamster ovary (CHO) cells are the predominant production vehicle for biotherapeutics. Quantitative proteomics data were obtained from two CHO cell lines (CHO-S and CHO DG44) and compared with seven Chinese hamster (Cricetulus griseus) tissues (brain, heart, kidney, liver, lung, ovary and spleen) by tandem mass tag (TMT) labeling followed by mass spectrometry, providing a comprehensive hamster tissue and cell line proteomics atlas. Of the 8470 unique proteins identified, high similarity was observed between CHO-S and CHO DG44 and included increases in proteins involved in DNA replication, cell cycle, RNA processing, and chromosome processing. Alternatively, gene ontology and pathway analysis in tissues indicated increased protein intensities related to important tissue functionalities. Proteins enriched in the brain included those involved in acidic amino acid metabolism, Golgi apparatus, and ion and phospholipid transport. The lung showed enrichment in proteins involved in BCAA catabolism, ROS metabolism, vesicle trafficking, and lipid synthesis while the ovary exhibited enrichments in extracellular matrix and adhesion proteins. The heart proteome included vasoconstriction, complement activation, and lipoprotein metabolism enrichments. These detailed comparisons of CHO cell lines and hamster tissues will enhance understanding of the relationship between proteins and tissue function and pinpoint potential pathways of biotechnological relevance for future cell engineering. |
| Databáze: | OpenAIRE |
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