Effects of earthworms on the fungal community and microbial activity in root-adhering soil of Lantana camara during phytoextraction of lead

Autor: Franck Poly, Thierry Lebeau, Corinne Rouland-Lefèvre, Edouard Miambi, My Dung Jusselme
Přispěvatelé: Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Laboratoire de Planétologie et Géodynamique UMR6112 (LPG), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Université d'Angers (UA), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Laboratoire de Planétologie et Géodynamique [UMR 6112] (LPG), Université d'Angers (UA)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Developpement (IRD), France
Rok vydání: 2015
Předmět:
Zdroj: Applied Soil Ecology
Applied Soil Ecology, Elsevier, 2015, 96, pp.151-158. ⟨10.1016/j.apsoil.2015.07.011⟩
ISSN: 0929-1393
Popis: International audience; Earthworms are known to stimulate soil microorganisms and to enhance phytoextraction of metals. This study was designed to test the hypothesis that the positive effect of earthworms on phytoextraction performance results from the stimulation of soil fungi that are known to be tolerant to heavy metals in contaminated soils. It set out to assess the relationships between lead phytoextraction and the changes in soil fungi in response to earthworm activity. The experiment was performed in outdoor microcosms combining: earthworms (Pontoscolex corethrurus), Spanish Flag (Lantana camara) and soil spiked with 500 and 1000 mg Pb kg(-1). Cultivable fungi were enumerated using plate counts. PCR-DGGE analysis targeting the 28S rRNA gene was used to determine the structure of the fungal community. Total microbial activity was measured by fluorescein diacetate (FDA) hydrolysis assay. Plant Pb uptake by L. camara increased by about 2.5-fold in the presence of earthworms, regardless of the Pb concentration. This was significantly correlated with the increase in total microbial activity, abundance of cultivable fungi and richness index of the fungal community calculated from DGGE banding patterns. In the presence of earthworms, there was a significant positive correlation between microbial activity, plant biomass and plant Pb uptake. There was no correlation for the control without earthworms. These results provide evidence that interactions between earthworms and soil microorganisms have a positive effect on Pb-phytoextraction yield. The study of the ecological context of phytoremediation should be broadened by considering the interactions between plants, microorganisms and earthworms that affect heavy metal uptake by plants. (C) 2015 Elsevier B.V. All rights reserved.
Databáze: OpenAIRE
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