Microsatellite typing of Aspergillus flavus from clinical and environmental avian isolates
| Autor: | I. Drira, Fattouma Makni, Inès Hadrich, Stéphane Ranque, Sourour Neji, Ali Ayadi, Nedia Mahfoud |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Genotype Veterinary Microbiology Aspergillus flavus Biology Aspergillosis Microbiology Aspergillus fumigatus Egg Shell Environmental Microbiology medicine Animals Typing DNA Fungal Alleles Phylogeny Ovum Bird Diseases General Medicine medicine.disease biology.organism_classification Penicillium Food Microbiology Microsatellite Pulmonary Aspergillosis Chickens Microsatellite Repeats Cladosporium |
| Zdroj: | Journal of Medical Microbiology. 62:121-125 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.047803-0 |
| Popis: | Aspergillosis is one of the most common causes of death in captive birds. Aspergillus fumigatus accounts for approximately 95 % of aspergillosis cases and Aspergillus flavus is the second most frequent organism associated with avian infections. In the present study, the fungi were grown from avian clinical samples (post-mortem lung material) and environmental samples (eggs, food and litter). Microsatellite markers were used to type seven clinical avian isolates and 22 environmental isolates of A. flavus. A. flavus was the only species (28 % prevalence) detected in the avian clinical isolates, whereas this species ranked third (19 %) after members of the genera Penicillium (39 %) and Cladosporium (21 %) in the environmental samples. Upon microsatellite analysis, five to eight distinct alleles were detected for each marker. The marker with the highest discriminatory power had eight alleles and a 0.852 D value. The combination of all six markers yielded a 0.991 D value with 25 distinct genotypes. One clinical avian isolate (lung biopsy) and one environmental isolate (egg) shared the same genotype. Microsatellite typing of A. flavus grown from avian and environmental samples displayed an excellent discriminatory power and 100 % reproducibility. This study showed a clustering of clinical and environmental isolates, which were clearly separated. Based upon these results, aspergillosis in birds may be induced by a great diversity of isolates. |
| Databáze: | OpenAIRE |
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