Identification of a novel fibronectin-aggrecan complex in the synovial fluid of knees with painful meniscal injury
| Autor: | S. Raymond Golish, Lewis S. Hanna, Robert Bowser, Jason M. Cuellar, Frank F. Cook, Gaetano J. Scuderi |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male medicine.medical_specialty Enzyme-Linked Immunosorbent Assay Knee Injuries Asymptomatic Menisci Tibial Arthroscopy Postoperative Complications Osteoarthritis Synovial Fluid medicine Synovial fluid Humans Orthopedics and Sports Medicine Aggrecans Prospective cohort study Aggrecan Pain Measurement medicine.diagnostic_test business.industry Cartilage Magnetic resonance imaging General Medicine Middle Aged Prognosis Surgery Fibronectins Tibial Meniscus Injuries medicine.anatomical_structure Knee pain Orthopedic surgery Female medicine.symptom business Biomarkers |
| Zdroj: | The Journal of bone and joint surgery. American volume. 93(4) |
| ISSN: | 1535-1386 |
| Popis: | Background: Molecular biomarkers associated with knee pain may be useful as diagnostic modalities, prognostic indicators, and surrogate end points for therapeutic trials. The present study describes a novel complex of fibronectin and aggrecan that is present in the affected knee of patients with pain and meniscal abnormality. Methods: The present prospective study included thirty patients with knee pain, mechanical symptoms, and magnetic resonance imaging findings that were positive for a meniscal tear who chose arthroscopic partial meniscectomy after unsuccessful nonoperative management. Synovial fluid was aspirated at the time of surgery and was assayed for the fibronectin-aggrecan complex with use of a heterogeneous enzyme-linked immunosorbent assay (ELISA). The results were compared with knee aspirates from ten asymptomatic volunteers with no pain who underwent magnetic resonance imaging of the knee. Results: The mean optical density (and standard deviation) of the fibronectin-aggrecan complex was significantly greater in synovial fluid from knees undergoing arthroscopic surgery as compared with fluid from asymptomatic controls (13.29 ± 8.48 compared with 0.03 ± 0.09; p < 0.001). The mean age in the study group was significantly greater than in control group (46.0 ± 12.6 compared with 38.5 ± 6.0 years; p = 0.02), but controlling for age did not affect the results. Post hoc, an optical density cutoff value of 0.3 distinguished the study group from the control group with 100% accuracy. Conclusions: A novel fibronectin-aggrecan complex is present in the synovial fluid of painful knees with meniscal abnormality. The fibronectin-aggrecan complex may prove to be useful as a clinical biomarker or therapeutic target. Further research is warranted to correlate functional outcome after surgery with the fibronectin-aggrecan complex and other cartilage biomarkers. Level of Evidence: Diagnostic Level IV. See Instructions to Authors for a complete description of levels of evidence. |
| Databáze: | OpenAIRE |
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