A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent’s Brain
| Autor: | Mircea Dumitru Croitoru, Bianca-Eugenia Ősz, George Jîtcă, Mădălina-Georgiana Bătrînu, Amelia Tero-Vescan, Erzsébet Fogarasi, Camil Eugen Vari, Amalia Miklos, Carmen Maria Rusz, Maria Titica Dogaru |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
malondialdehyde
Acetonitriles brain Pharmaceutical Science Organic chemistry High-performance liquid chromatography Article Analytical Chemistry chemistry.chemical_compound QD241-441 Chromatography detector Drug Discovery Animals Humans oxidative stress liquid chromatography Physical and Theoretical Chemistry diode array detector Chromatography High Pressure Liquid Isocratic elution Chromatography Elution Phosphate buffered saline Malondialdehyde Metformin Rats Chromatographic separation chemistry Chemistry (miscellaneous) Haloperidol Molecular Medicine Hplc dad |
| Zdroj: | Molecules Volume 26 Issue 16 Molecules, Vol 26, Iss 5066, p 5066 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26165066 |
| Popis: | In the present study, a HPLC/DAD method was set up to allow for the determination and quantification of malondialdehyde (MDA) in the brain of rodents (rats). Chromatographic separation was achieved on Supelcosil LC-18 (3 μm) SUPELCO Column 3.3 cm × 4.6 mm and Supelco Column Saver 0.5 μm filter by using a mobile phase acetonitrile (A) and phosphate buffer (20 mM, pH = 6) (B). Isocratic elution was 14% for (A) and 86% for (B). The injection volume (loop mode) was 100 μL with an analysis time of 1.5 min. Flow rate was set at 1 mL/min. The eluted compound was detected at 532 nm by a DAD detector by keeping the column oven at room temperature. The results indicated that the method has good linearity in the range of 0.2–20 μg/g. Both intra- and inter-day precision, expressed as RSD, were ≤15% and the accuracies ranged between ±15%. The lower limit of quantification (LLOQ), stability, and robustness were evaluated and satisfied the validation criteria. The method was successfully applied in a study of chronic toxicology following different treatment regimens with haloperidol and metformin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|