Crystallization and identification of a binary complex of a elastase-I and a Carboxypeptidase Aγ from porcine pancreas
| Autor: | Atsushi Koide, Yoshizawa Masayuki |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Autolysis (biology)
Carboxypeptidases A Macromolecular Substances Swine Sequence analysis Biophysics Carboxypeptidases Biochemistry Elastase I law.invention Column chromatography Multienzyme Complexes law Animals Binary complex Porcine pancreas Crystallization Pancreas Molecular Biology Chromatography Pancreatic Elastase biology Chemistry Cell Biology Carboxypeptidase biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications. 100:1091-1098 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(81)91935-5 |
| Popis: | A binary complex of elastase I and Carboxypeptidase Aγ has been isolated and crystallized from activated extracts of porcine pancreas. The purification procedure included ammonium sulfate fractionation and autolysis treatment followed by crystallization. The two components in the complex have been separated by DEAE-Sephadex A-50 column chromatography to homogeneity and their identification was demonstrated by NH 2 -terminal sequence analysis. An analysis of the reconstitution crystal from the mixtures of both components showed that the complex consisted of a 1:1 molar ratio with elastase I and Carboxypeptidase Aγ, forming a binary complex in crystalline state. |
| Databáze: | OpenAIRE |
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