Dual color optogenetic control of neural populations using low-noise, multishank optoelectrodes
Autor: | Mihály Vöröslakos, Eran Stark, Euisik Yoon, Sam McKenzie, John P. Seymour, György Buzsáki, Daniel F. English, Kensall D. Wise, Komal Kampasi |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Materials science Materials Science (miscellaneous) Optogenetics lcsh:Technology Waveguide (optics) Noise (electronics) Industrial and Manufacturing Engineering Article law.invention 03 medical and health sciences 0302 clinical medicine law Biological neural network Electrical and Electronic Engineering Diode lcsh:T business.industry Condensed Matter Physics Laser Atomic and Molecular Physics and Optics Coupling (electronics) 030104 developmental biology lcsh:TA1-2040 Temporal resolution Optoelectronics lcsh:Engineering (General). Civil engineering (General) business 030217 neurology & neurosurgery |
Zdroj: | Microsystems & Nanoengineering Microsystems & Nanoengineering, Vol 4, Iss 1, Pp 1-16 (2018) |
Popis: | Optogenetics allows for optical manipulation of neuronal activity and has been increasingly combined with intracellular and extracellular electrophysiological recordings. Genetically-identified classes of neurons are optically manipulated, though the versatility of optogenetics would be increased if independent control of distinct neural populations could be achieved on a sufficient spatial and temporal resolution. We report a scalable multisite optoelectrode design that allows simultaneous optogenetic control of two spatially intermingled neuronal populations in vivo. We describe the design, fabrication, and assembly of low-noise, multisite/multicolor optoelectrodes. Each shank of the four-shank assembly is monolithically integrated with 8 recording sites and a dual-color waveguide mixer with a 7 × 30 μm cross-section, coupled to 405 nm and 635 nm injection laser diodes (ILDs) via gradient-index (GRIN) lenses to meet optical and thermal design requirements. To better understand noise on the recording channels generated during diode-based activation, we developed a lumped-circuit modeling approach for EMI coupling mechanisms and used it to limit artifacts to amplitudes under 100 μV upto an optical output power of 450 μW. We implanted the packaged devices into the CA1 pyramidal layer of awake mice, expressing Channelrhodopsin-2 in pyramidal cells and ChrimsonR in paravalbumin-expressing interneurons, and achieved optical excitation of each cell type using sub-mW illumination. We highlight the potential use of this technology for functional dissection of neural circuits. |
Databáze: | OpenAIRE |
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