A multiple ion-uptake phenotyping platform reveals shared mechanisms affecting nutrient uptake by roots
| Autor: | Haichao Guo, Larry M. York, Yaxin Ge, Robert E. Sharp, Marcus Griffiths, Anand Seethepalli, Sonali Roy, Felix B. Fritschi, David V. Huhman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Crops Agricultural Regular Issue Genotype Physiology Population Plant Science Biology 01 natural sciences Plant Roots Zea mays Crop 03 medical and health sciences chemistry.chemical_compound Nutrient Nitrate Respiration Genetics Ammonium Nested association mapping education 030304 developmental biology 0303 health sciences education.field_of_study Ion Transport Genetic Variation Phenotype chemistry Agronomy Respiration rate 010606 plant biology & botany |
| Zdroj: | Plant Physiol |
| Popis: | Nutrient uptake is critical for crop growth and is determined by root foraging in soil. Growth and branching of roots lead to effective root placement to acquire nutrients, but relatively little is known about absorption of nutrients at the root surface from the soil solution. This knowledge gap could be alleviated by understanding sources of genetic variation for short-term nutrient uptake on a root length basis. A modular platform called RhizoFlux was developed for high-throughput phenotyping of multiple ion-uptake rates in maize (Zea mays L.). Using this system, uptake rates were characterized for the crop macronutrients nitrate, ammonium, potassium, phosphate, and sulfate among the Nested Association Mapping (NAM) population founder lines. The data revealed substantial genetic variation for multiple ion-uptake rates in maize. Interestingly, specific nutrient uptake rates (nutrient uptake rate per length of root) were found to be both heritable and distinct from total uptake and plant size. The specific uptake rates of each nutrient were positively correlated with one another and with specific root respiration (root respiration rate per length of root), indicating that uptake is governed by shared mechanisms. We selected maize lines with high and low specific uptake rates and performed an RNA-seq analysis, which identified key regulatory components involved in nutrient uptake. The high-throughput multiple ion-uptake kinetics pipeline will help further our understanding of nutrient uptake, parameterize holistic plant models, and identify breeding targets for crops with more efficient nutrient acquisition. |
| Databáze: | OpenAIRE |
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