The Impact of gamma-Irradiation and EtO Degassing on Tissue Remodeling of Collagen-based Hybrid Tubular Templates
| Autor: | Wout F.J. Feitz, Heinz P. Janke, Paul K J D de Jonge, Silvia M. Mihaila, Egbert Oosterwijk, Dorien M. Tiemessen, Marije Sloff, Barbara B.M. Kortmann, Paul J. Geutjes |
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| Přispěvatelé: | Biomedische Technologie, RS: CARIM - R2.09 - Cardiovascular system dynamics |
| Rok vydání: | 2018 |
| Předmět: |
Biocompatibility
Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2] collagen type I 030232 urology & nephrology Biomedical Engineering animal model (goat) 02 engineering and technology Article BIOCOMPATIBILITY Biomaterials 03 medical and health sciences 0302 clinical medicine All institutes and research themes of the Radboud University Medical Center ETHYLENE-OXIDE STERILIZATION Tissue engineering TERMINAL STERILIZATION BIOMATERIAL In vivo Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15] STRENGTH Vicryl ARCHITECTURE Chemistry SCAFFOLD Biomaterial Terminal Sterilization sterilization Sterilization (microbiology) DEGRADATION 021001 nanoscience & nanotechnology 3. Good health Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] tensile strength tissue remodeling PHASE-II 0210 nano-technology Type I collagen polyglactin 910 (Vicryl) BEHAVIOR Biomedical engineering |
| Zdroj: | Acs Biomaterials Science & Engineering, 4, 3282-3290 Acs Biomaterials Science & Engineering, 4, 9, pp. 3282-3290 ACS Biomaterial Science and Engineering, 4(9), 3282-3290. American Chemical Society ACS Biomaterials Science & Engineering |
| ISSN: | 3282-3290 2373-9878 |
| Popis: | Clinical implementation of novel products for tissue engineering and regenerative medicine requires a validated sterilization method. In this study, we investigated the effect of gamma-irradiation and EtO degassing on material characteristics in vitro and the effect on template remodeling of hybrid tubular constructs in a large animal model. Hybrid tubular templates were prepared from type I collagen and Vicryl polymers and sterilized by 25 kGray of gamma-irradiation or EtO degassing. The in vitro characteristics were extensively studied, including tensile strength analysis and degradation studies. For in vivo evaluation, constructs were subcutaneously implanted in goats for 1 month to form vascularized neotissue. Macroscopic and microscopic appearances of the gamma - and EtO-sterilized constructs slightly differed due to additional processing required for the COL-Vicryl-EtO constructs. Regardless of the sterilization method, incubation in urine resulted in fast degradation of the Vicryl polymer and decreased strength ( |
| Databáze: | OpenAIRE |
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