Soft Hydrogel Inspired by Elastomeric Proteins
| Autor: | Brigida Bochicchio, Lucia Maio, Angelo Bracalello, Alessandra Girotti, Francisco Javier Arias, Antonietta Pepe, Luis Quintanilla-Sierra |
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| Rok vydání: | 2021 |
| Předmět: |
cytocompatibility
Circular dichroism biology Tropoelastin Biomedical Engineering elastin Trimer Sequence (biology) Hydrogels Article circular dichroism Biomaterials chemistry.chemical_compound Monomer chemistry antiadhesive materials biology.protein Biophysics Collagen hydrogel Resilin Peptides Gene Elastin |
| Zdroj: | ACS Biomaterials Science & Engineering |
| ISSN: | 2373-9878 |
| Popis: | Elastin polypeptides based on -VPGVG- repeated motifs are widely used in the production of biomaterials because they are stimuli-responsive systems. On the other hand, glycine-rich sequences, mainly present in tropoelastin terminal domains, are responsible for the elastin self-assembly. In a previous study, we have recombinantly expressed a chimeric polypeptide, named resilin, elastin, and collagen (REC), inspired by glycine-rich motifs of elastin and containing resilin and collagen sequences as well. Herein, a three-block polypeptide, named (REC)3, was expressed starting from the previous monomer gene by introducing key modifications in the sequence. The choice was mandatory because the uneven distribution of the cross-linking sites in the monomer precluded the hydrogel production. In this work, the cross-linked polypeptide appeared as a soft hydrogel, as assessed by rheology, and the linear un-cross-linked trimer self-aggregated more rapidly than the REC monomer. The absence of cell-adhesive sequences did not affect cell viability, while it was functional to the production of a material presenting antiadhesive properties useful in the integration of synthetic devices in the body and preventing the invasion of cells. |
| Databáze: | OpenAIRE |
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