Isolation of the murine branched-chain α-ketoacid dehydrogenase E2 subunit promoter region
| Autor: | Paul A. Costeas, Jeffrey M. Chinsky |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA
Complementary Base Sequence Chemistry Sequence analysis Protein subunit Molecular Sequence Data Biophysics CAAT box Ketone Oxidoreductases Promoter Dehydrogenase Biochemistry Molecular biology 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) Mice Multienzyme Complexes Structural Biology Genetics Animals DNA Probes Gene Gene Library Sequence (medicine) Minigene |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1399:111-116 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(98)00084-0 |
| Popis: | The promoter region of the murine branched-chain α-ketoacid dehydrogenase E2 subunit (dihydrolipoyl transacylase) gene was isolated and characterized. Sequence analysis of the promoter-regulatory region showed the presence of two inverted ‘CAAT box’ sequences, the most proximal being −42 to −48 bp upstream from the determined transcription initiation site, but no TATA-box sequences, similar to the human BCKAD E2 gene. The boundary of the minimum promoter sequence appeared to reside just inclusive of this first inverted CAAT sequence, but minigene transfer analysis demonstrated that the promoter proximal region between −65 and −140 bp is likely to be extremely important for controlling regulated changes in E2 RNA expression. The regulatory effect of this region may be modulated by a number of other upstream regions which were identified within the 7.0 kb sequence examined. |
| Databáze: | OpenAIRE |
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