ASB2 Is an Elongin BC-interacting Protein That Can Assemble with Cullin 5 and Rbx1 to Reconstitute an E3 Ubiquitin Ligase Complex
| Autor: | Pierre G. Lutz, Yvon E. Cayre, Joan W. Conaway, Arndt Benecke, Florence C. Guibal, Charles A.S. Banks, Mélina L. Heuzé, Ronald C. Conaway |
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| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) |
| Rok vydání: | 2005 |
| Předmět: |
Acute promyelocytic leukemia
Proteasome Endopeptidase Complex Protein family Ubiquitin-Protein Ligases [SDV]Life Sciences [q-bio] RBX1 Elongin Biochemistry Cell Line 03 medical and health sciences DDB1 0302 clinical medicine Ubiquitin Two-Hybrid System Techniques medicine Animals Humans Molecular Biology Adaptor Proteins Signal Transducing 030304 developmental biology 0303 health sciences Leukemia biology Cell Biology Cullin Proteins medicine.disease Molecular biology Ubiquitin ligase Proteasome Multiprotein Complexes 030220 oncology & carcinogenesis Mutation biology.protein Carrier Proteins Protein Processing Post-Translational Cullin Protein Binding Transcription Factors |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2005, 280 (7), pp.5468-5474. ⟨10.1074/jbc.M413040200⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.m413040200 |
| Popis: | International audience; The ankyrin repeat-containing protein with a suppressor of cytokine signaling box-2 (ASB2) gene was identified as a retinoic acid-response gene and a target of the promyelocytic leukemia-retinoic acid receptor-alpha oncogenic protein characteristic of acute promyelocytic leukemia. Expression of ASB2 in myeloid leukemia cells inhibits growth and promotes commitment, recapitulating an early step known to be critical for differentiation. Here we show that ASB2, by interacting with the Elongin BC complex, can assemble with Cullin5.Rbx1 to form an E3 ubiquitin ligase complex that stimulates polyubiquitination by the E2 ubiquitin-conjugating enzyme Ubc5. This is a first indication that a member of the ASB protein family, ASB2, is a subunit of an ECS (Elongin C-Cullin-SOCS box)-type E3 ubiquitin ligase complex. Altogether, our results strongly suggest that ASB2 targets specific proteins to destruction by the proteasome in leukemia cells that have been induced to differentiate. |
| Databáze: | OpenAIRE |
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