Intrinsic expression of transcortin in neural cells of the mouse brain: a histochemical and molecular study
| Autor: | Elena V. Sivukhina, Véronique Pallet, Hendrick H. Schäfer, Jean-Christophe Helbling, Amandine M. Minni, Marie-Pierre Moisan, Gustav F. Jirikowski |
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| Přispěvatelé: | Friedrich-Schiller-Universität = Friedrich Schiller University Jena [Jena, Germany], Nutrition et Neurobiologie intégrée (NutriNeuro), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), We wish to thank The Journal of Experimental Biology and The Company of Biologists Ltd (Travelling Fellowship), and ‘Boehringer Ingelheim Foundation’ for their financial support to E.S. for part of this work. |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Cerebellum DNA Complementary corticosteroid-binding globulin Physiology brain [SDV]Life Sciences [q-bio] Gene Expression Aquatic Science 03 medical and health sciences Mice 0302 clinical medicine Transcortin Internal medicine Gene expression medicine Animals RNA Messenger Molecular Biology Ecology Evolution Behavior and Systematics Cellular localization 030304 developmental biology Brain Chemistry Mice Knockout 0303 health sciences Messenger RNA biology Histocytochemistry glial cell 3. Good health medicine.anatomical_structure Endocrinology Cerebral cortex Insect Science Knockout mouse biology.protein Animal Science and Zoology Choroid plexus Female glucocorticoid transcortin 030217 neurology & neurosurgery |
| Zdroj: | Journal of Experimental Biology Journal of Experimental Biology, The Company of Biologists, 2013, 216 (2), pp.245-252. ⟨10.1242/jeb.076893⟩ |
| ISSN: | 0022-0949 1477-9145 |
| DOI: | 10.1242/jeb.076893⟩ |
| Popis: | Summary Corticosteroid binding globulin (CBG, transcortin) has been shown to be expressed in the brain of rat and human species. In this study we examined the CBG brain expression and cDNA structure in mice, comparing wild-type (Cbg+/+) and Cbg knockout mice (Cbg-/-, obtained by genetic disruption of the SerpinA6 alias Cbg gene). We used double immunofluorescence labelling with specific neuronal and glial markers to analyze the cellular localization of CBG in various regions of the mouse brain. In wild-type (Cbg+/+) mice we found CBG immunoreactivity in neuronal perikarya of the magnocellular hypothalamic nuclei, amygdala, hippocampus, cerebral cortex, cerebellum and pituitary. A portion of glial cells (astrocytes, oligodendrocytes) contained CBG immunoreactivity, including some of the ependymal cells and choroid plexus cells. No CBG immunoreactivity was detected in Cbg-/- brain tissues. We showed by RT-PCR that the full-length Cbg mRNA is present in those regions, indicating an intrinsic expression of the steroid-binding globulin. Furthermore, we found by sequencing analysis that Cbg cDNA obtained from the mouse hypothalamus was homologous to Cbg cDNA obtained from the liver. Finally, we have evaluated the relative levels of CBG expression by quantitative PCR in various brain regions and in the liver. We found that brain levels of Cbg mRNA are low compared to the liver but significantly higher than in CBG-deficient mice. Although derived from the same gene than liver CBG, brain CBG protein may play a specific or complementary role that requires the production and analysis of brain-specific Cbg knockout models. |
| Databáze: | OpenAIRE |
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