Experimental mixed infection ofLeishmania(Leishmania)amazonensisandLeishmania(L.)infantumin hamsters (Mesocricetus auratus)
| Autor: | Hélida Monteiro de Andrade, Gabriela Matos de Sousa, Maria Norma Melo, Ana Paula Venuto Moura, João Carlos França-Silva, Carolina Carvalho de Souza, Jordanna Luíza De Lima Celeste, Soraia de Oliveira Silva, Wagner Luiz Tafuri |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Blotting Western 030231 tropical medicine Hamster Microbiology 03 medical and health sciences 0302 clinical medicine Immune system Western blot Cricetinae parasitic diseases medicine Animals Leishmania infantum Leishmaniasis Leishmania Mesocricetus biology medicine.diagnostic_test Coinfection Immunogenicity South America biology.organism_classification medicine.disease Immunity Humoral Disease Models Animal 030104 developmental biology Infectious Diseases Visceral leishmaniasis Leishmaniasis Visceral Animal Science and Zoology Parasitology Spleen |
| Zdroj: | Parasitology. 144:1191-1202 |
| ISSN: | 1469-8161 0031-1820 |
| Popis: | SUMMARYIn South America, visceral leishmaniasis is frequently caused byLeishmania infantumand, at an unknown frequency, byLeishmania amazonensis. Therefore, mixed infections with these organisms are possible. Mixed infections might affect the clinical course, immune response, diagnosis, treatment and epidemiology of the disease. Here we describe the clinical course of mixed infections withL. amazonensisandL. infantumin a hamster model. We show that mixed infections are associated with more severe clinical disease than infection withL. amazonensisorL. infantumalone. In spleens with mixed infections,L. infantumoutcompetedL. amazonensisin the tissue, but not in culture from tissue. We found increased levels of IgG in animals infected withL. infantum.Although more than 30 bands were revealed in a Western blot, the highest immunogenicity was observed with proteins having molecular masses of 95 and 90 kDa, whereas proteins with molecular masses of lower than 50 kDa were reactive frequently with serum from hamsters infected withL. amazonensis, and proteins with molecular masses of 80 and 70 kDa were reactive only with serum from hamsters infected withL. infantum. This finding has important implications regarding the biology ofLeishmaniaand humoral immune responses to infections with these organisms. |
| Databáze: | OpenAIRE |
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