Thiamine Status in Humans and Content of Phosphorylated Thiamine Derivatives in Biopsies and Cultured Cells

Autor: Frédéric Chantraine, Marjorie Gangolf, Caroline Jouan, Michelle Nisolle, Marc Radermecker, Didier Martin, Olivier Detry, Pierre Wins, Thierry Grisar, Bernard Lakaye, Jan Czerniecki, Lucien Bettendorff
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Vitamin
medicine.medical_specialty
Developmental Biology/Germ Cells
Biopsy
Geriatrics/Dementia
lcsh:Medicine
Adenosine thiamine triphosphate
Neurological Disorders
Cofactor
chemistry.chemical_compound
Mice
Thiamine triphosphatase
Internal medicine
medicine
Animals
Humans
Thiamine
Phosphorylation
lcsh:Science
Cells
Cultured
Chromatography
High Pressure Liquid
chemistry.chemical_classification
Multidisciplinary
Evidence-Based Healthcare
biology
Reverse Transcriptase Polymerase Chain Reaction
lcsh:R
Physiology/Cardiovascular Physiology and Circulation
Biochemistry/Chemical Biology of the Cell
food and beverages
Thiamine monophosphate
Cell Biology
Nutrition/Deficiencies
Body Fluids
Rats
Endocrinology
Enzyme
Biochemistry
chemistry
biology.protein
lcsh:Q
Thiamine triphosphate
human activities
Oxidation-Reduction
Research Article
Zdroj: PLoS ONE
PLoS ONE, Vol 5, Iss 10, p e13616 (2010)
ISSN: 1932-6203
Popis: Background Thiamine (vitamin B1) is an essential molecule for all life forms because thiamine diphosphate (ThDP) is an indispensable cofactor for oxidative energy metabolism. The less abundant thiamine monophosphate (ThMP), thiamine triphosphate (ThTP) and adenosine thiamine triphosphate (AThTP), present in many organisms, may have still unidentified physiological functions. Diseases linked to thiamine deficiency (polyneuritis, Wernicke-Korsakoff syndrome) remain frequent among alcohol abusers and other risk populations. This is the first comprehensive study on the distribution of thiamine derivatives in human biopsies, body fluids and cell lines. Methodology and Principal Findings Thiamine derivatives were determined by HPLC. In human tissues, the total thiamine content is lower than in other animal species. ThDP is the major thiamine compound and tissue levels decrease at high age. In semen, ThDP content correlates with the concentration of spermatozoa but not with their motility. The proportion of ThTP is higher in humans than in rodents, probably because of a lower 25-kDa ThTPase activity. The expression and activity of this enzyme seems to correlate with the degree of cell differentiation. ThTP was present in nearly all brain and muscle samples and in ∼60% of other tissue samples, in particular fetal tissue and cultured cells. A low ([ThTP]+[ThMP])/([Thiamine]+[ThMP]) ratio was found in cardiovascular tissues of patients with cardiac insufficiency. AThTP was detected only sporadically in adult tissues but was found more consistently in fetal tissues and cell lines. Conclusions and Significance The high sensitivity of humans to thiamine deficiency is probably linked to low circulating thiamine concentrations and low ThDP tissue contents. ThTP levels are relatively high in many human tissues, as a result of low expression of the 25-kDa ThTPase. Another novel finding is the presence of ThTP and AThTP in poorly differentiated fast-growing cells, suggesting a hitherto unsuspected link between these compounds and cell division or differentiation.
Databáze: OpenAIRE
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