Helicobacter bilis sp. nov., a novel Helicobacter species isolated from bile, livers, and intestines of aged, inbred mice
| Autor: | Brian D. Shames, James C. Murphy, J C Belcher, L Yan, Alison Hayward, James G. Fox, Bruce J. Paster, Floyd E. Dewhirst, E N Mendes |
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| Rok vydání: | 1995 |
| Předmět: |
DNA
Bacterial Male Microbiology (medical) Aging Helicobacter bilis Helicobacter pullorum Nalidixic acid Molecular Sequence Data Hepatitis Animal Polymerase Chain Reaction Microbiology Mice Species Specificity Helicobacter RNA Ribosomal 16S medicine Animals Bile Phylogeny DNA Primers Mice Inbred BALB C Oxidase test Base Sequence biology Helicobacter trogontum biology.organism_classification Intestines Mice Inbred C57BL Microscopy Electron RNA Bacterial Liver Mice Inbred DBA Mice Inbred CBA Female Helicobacter hepaticus Bacteria Research Article medicine.drug |
| Zdroj: | Journal of Clinical Microbiology. 33:445-454 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | A fusiform bacterium with 3 to 14 multiple bipolar sheathed flagella and periplasmic fibers wrapped around the cell was isolated from the liver, bile, and lower intestine of aged, inbred mice. The bacteria grew at 37 and 42 degrees C under microaerophilic conditions, rapidly hydrolyzed urea, were catalase and oxidase positive, reduced nitrate to nitrite, did not hydrolyze indoxyl acetate or hippurate, and were resistant to both cephalothin and nalidixic acid but sensitive to metronidazole. On the basis of 16S rRNA gene sequence analysis, the organism was classified as a novel helicobacter, Helicobacter bilis. This new helicobacter, like Helicobacter hepaticus, colonizes the bile, liver, and intestine of mice. Although the organism is associated with multifocal chronic hepatitis, further studies are required to ascertain whether H. bilis is responsible for causing chronic hepatitis and/or hepatocellular tumors in mice. |
| Databáze: | OpenAIRE |
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