Effects of extender type on the quality of post-thaw giant panda (Ailuropoda melanoleuca) semen
| Autor: | Yuliang Liu, Li Yuan, Ling He, Shangmian Yie, Wang Donghui, Jia-Song Chen, Jingchao Lan, An Junhui, He Ping, Zhang Ying, Cai Zhigang, Rong Hou, Zhihe Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
media_common.quotation_subject Semen General Biochemistry Genetics and Molecular Biology Cryopreservation law.invention Andrology 03 medical and health sciences Semen quality 0302 clinical medicine Human fertilization Cryoprotective Agents law biology.animal Animals Ailuropoda melanoleuca media_common 030219 obstetrics & reproductive medicine biology urogenital system Extender 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Semen cryopreservation Spermatozoa Semen Analysis Reproduction General Agricultural and Biological Sciences Ursidae Semen Preservation |
| Zdroj: | Cryobiology. 94 |
| ISSN: | 1090-2392 |
| Popis: | Sperm cryopreservation is an essential approach for assisted reproduction and genetic resources conservation in captive giant pandas. Cryopreservation, however, leads to a significant decrease in sperm quality and, consequently, a low fertilization rate. Therefore, it is mandatory to disclose more suitable and efficient freezing strategies for sperm cryopreservation. In the present study, we compared for the first time the performance of two commercial freeze extender (INRA96 versus TEST) freezing methods on post-thawed semen quality. Semen cryopreserved with the INRA96 showed better total motility (73.00 ± 4.84% vs 57.56 ± 3.60%, P 0.001), membrane integrity (60.92 ± 2.27% vs 40.53 ± 2.97%, P 0.001) and acrosome integrity (90.39 ± 2.74% vs 84.26 ± 4.27%, P 0.05) than stored with TEST. There was no significant difference in DNA integrity after thawing between the two extenders (95.69 ± 3.60% vs 94.26 ± 4.84%). In conclusion, the INRA96 method showed to be better for giant panda sperm cryopreservation and should therefore be recommended for use in order to increase success of artificial insemination. |
| Databáze: | OpenAIRE |
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