Characterization of a sigma class GST (GSTS6) required for cellular detoxification and embryogenesis inTribolium castaneum
Autor: | Yuemei Zhang, Bin Li, Fan Feng, Yan-Hao Ji, Jing Tang, Xiaowen Song, Qisheng Zhong, Jia Xie, Jingxiu Bi |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Insecticides Antioxidant medicine.medical_treatment Cellular detoxification Embryonic Development 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound RNA interference medicine Animals Phylogeny Ecology Evolution Behavior and Systematics Glutathione Transferase Tribolium Gene knockdown biology fungi Glutathione 010602 entomology 030104 developmental biology Glutathione S-transferase chemistry Biochemistry Cumene hydroperoxide Insect Science Inactivation Metabolic biology.protein Agronomy and Crop Science Function (biology) |
Zdroj: | Insect Science. 29:215-229 |
ISSN: | 1744-7917 1672-9609 |
Popis: | The sigma glutathione S-transferases (GSTSs) are a class of cytosolic glutathione S transferases (GSTs) that play important roles in antioxidant defense in insects, but the mechanisms by which GSTSs contribute to antioxidant activity remain unclear. Here, we isolated a GSTS (GSTS6) from Tribolium castaneum and explored its function. Homology and phylogenetic analysis revealed that TcGSTS6 shared high identity with other evolutionarily conserved GSTSs. The recombinant TcGSTS6 protein had strong activity toward cumene hydroperoxide and 4-hydroxynonenal but low activity toward the universal substrate 1-chloro-2,4-dinitrobenzene. Exposure to various types of oxidative stress, including heat, cold, UV and pathogenic microbes, significantly induced TcGSTs6 expression, which indicates that it is involved in antioxidant defense. Knockdown TcGSTs6 by using RNA interference (RNAi) caused reduced antioxidant capacity, which was accomplished by cooperating with other antioxidant genes. Moreover, treatment with various insecticides such as phoxim, lambda-cyhalothrin, dichlorvos and carbofuran revealed that TcGSTS6 plays an important role in insecticide detoxification. The RNAi results showed that TcGSTS6 is essential for embryogenesis in T. castaneum. Our study elucidates the mechanism by which a GSTS contributes to antioxidant activity and enhances our understanding of the functional diversity of GSTSs in insects. |
Databáze: | OpenAIRE |
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