| Autor: |
Xiang Wu, Yuyan Jiang, Nicholas J. Rommelfanger, Fan Yang, Qi Zhou, Rongkang Yin, Junlang Liu, Sa Cai, Wei Ren, Andrew Shin, Kyrstyn S. Ong, Kanyi Pu, Guosong Hong |
| Přispěvatelé: |
School of Chemical and Biomedical Engineering, School of Physical and Mathematical Sciences |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Nature Biomedical Engineering. 6:754-770 |
| ISSN: |
2157-846X |
| Popis: |
Neural circuitry is typically modulated via invasive brain implants and tethered optical fibres in restrained animals. Here we show that wide-field illumination in the second near-infrared spectral window (NIR-II) enables implant-and-tether-free deep-brain stimulation in freely behaving mice with stereotactically injected macromolecular photothermal transducers activating neurons ectopically expressing the temperature-sensitive transient receptor potential cation channel subfamily V member 1 (TRPV1). The macromolecular transducers, ~40 nm in size and consisting of a semiconducting polymer core and an amphiphilic polymer shell, have a photothermal conversion efficiency of 71% at 1,064 nm, the wavelength at which light attenuation by brain tissue is minimized (within the 400-1,800 nm spectral window). TRPV1-expressing neurons in the hippocampus, motor cortex and ventral tegmental area of mice can be activated with minimal thermal damage on wide-field NIR-II illumination from a light source placed at distances higher than 50 cm above the animal's head and at an incident power density of 10 mW mm-2. Deep-brain stimulation via wide-field NIR-II illumination may open up opportunities for social behavioural studies in small animals. Ministry of Education (MOE) Nanyang Technological University G.H. acknowledges startup support from the Wu Tsai Neurosciences Institute of Stanford University, a National Institutes of Health (NIH) Pathway to Independence Award (National Institute on Aging 5R00AG056636-04), a National Science Foundation (NSF) CAREER Award (2045120), the Rita Allen Foundation Scholars Program, a gift from the Spinal Muscular Atrophy (SMA) Foundation, and seed grants from the Wu Tsai Neurosciences Institute and the Bio-X Initiative of Stanford University. X.W. acknowledges support from the Stanford Graduate Fellowship. K.S.O. acknowledges the NeuroTech training programme supported by the National Science Foundation under Grant No. 1828993. K.P. thanks Nanyang Technological University (startup grant: M4081627) and Singapore Ministry of Education Academic Research Fund Tier 2 (MOE2016-T2-1-098) for financial support. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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