Cloning theBamHI restriction modification system
| Autor: | Donald O. Nwankwo, Daniel F. Heiter, Laura A. Sznyter, Tina Jager-Quinton, Barton E. Slatko, Karen R. Silber, Jack S. Benner, Geoffrey G. Wilson, Laurie S. Moran, Joan E. Brooks |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Restriction Mapping Bacillus Restriction fragment Endonuclease Transformation Genetic Restriction map Plasmid Bacterial Proteins Escherichia coli Genetics Amino Acid Sequence Cloning Molecular DNA Modification Methylases Gene Rearrangement Base Sequence Deoxyribonuclease BamHI biology Gene rearrangement Molecular biology DNA Transposable Elements biology.protein Restriction modification system BamHI DNA Probes Plasmids |
| Zdroj: | Nucleic Acids Research. 17:979-997 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/17.3.979 |
| Popis: | BamHI, a Type II restriction modification system from Bacillus amyloliquefaciensH recognizes the sequence GGATCC. The methylase and endonuclease genes have been cloned into E. coli in separate steps; the clone is able to restrict unmodified phage. Although within the clone the methylase and endonuclease genes are present on the same pACYC184 vector, the system can be maintained in E. coli only with an additional copy of the methylase gene present on a separate vector. The initial selection for BamHI methylase activity also yielded a second BamHI methylase gene which is not homologous in DNA sequence and hybridizes to different genomic restriction fragments than does the endonuclease-linked methylase gene. Finally, the interaction of the BamHI system with the E. coli Dam and the Mcr A and B functions, have been studied and are reported here. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|