Peroxisome Proliferator-Activated Receptor Isoform Expression Changes in Human Gestational Tissues with Labor at Term
| Autor: | R. Stewart Gilmour, K.W. Marvin, Elicia B.E Berry, R.L. Eykholt, Murray D. Mitchell, Rachel J. A. Helliwell |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Placenta Receptors Cytoplasmic and Nuclear Peroxisome proliferator-activated receptor Biology Pregnancy Cell Line Tumor Internal medicine medicine Humans Protein Isoforms Amnion Northern blot Receptor reproductive and urinary physiology Pharmacology chemistry.chemical_classification Labor Obstetric Choriocarcinoma medicine.disease Endocrinology medicine.anatomical_structure chemistry Nuclear receptor Molecular Medicine Female lipids (amino acids peptides and proteins) Rosiglitazone Transcription Factors medicine.drug |
| Zdroj: | Molecular Pharmacology. 64:1586-1590 |
| ISSN: | 1521-0111 0026-895X |
| DOI: | 10.1124/mol.64.6.1586 |
| Popis: | Peroxisome proliferator-activated receptors (PPARs) are a family of nuclear receptors that are involved in lipid metabolism, differentiation, proliferation, cell death, and inflammation. Three subtypes have been identified: PPAR-alpha, -delta, and -gamma. We have previously shown presence of PPAR-gamma mRNA in the amnion, choriodecidua, and placenta, and its level of expression was unchanged with labor. To evaluate whether PPAR-alpha and -delta subtypes are present in intrauterine tissues, placentae were obtained from women at term after spontaneous vaginal delivery (TSL; n = 15) and elective caesarean section before labor (TNL; n = 15). Northern blot analyses were used to evaluate the mRNA for PPARs. Activities of PPARs were assessed using JEG3 choriocarcinoma cells transfected with a PPAR-response element reporter construct (pTK-PPREx3-luc) and treated with PPAR ligands. The PPAR-gamma-specific ligand rosiglitazone induced PPAR response element (PPRE)-mediated activity in a concentration-dependent manner, whereas the PPAR-gamma-specific irreversible inhibitor GW9662 fully inhibited this induction. However, GW9662 only partially inhibited 15-deoxy-Delta12,14-prostaglandin J2 (15d-PGJ2)-induced luciferase activity, suggesting that 15d-PGJ2 may also activate either of the other isoforms. PPAR-alpha and -delta are expressed in the amnion, choriodecidua, and placental villous tissues. In the amnion, although for PPAR-alpha no significant difference in expression was observed with labor, PPAR-delta expression increased significantly (p < 0.001). In the choriodecidua, expression of PPAR-alpha declined with labor (p < 0.01), whereas, as in the amnion, PPAR-delta expression increased (p < 0.05). In the placenta, both PPAR-alpha and -delta expression increased with labor (p < 0.005). The changes observed with labor suggest that regulation of PPAR expression and function may have roles to the mechanisms that maintain pregnancy or initiate labor. |
| Databáze: | OpenAIRE |
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