Modeling Immune Evasion and Vaccine Limitations by Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice
| Autor: | Laura K. Howard, Demba Sarr, Bodo Linz, Kalyan K. Dewan, Balázs Rada, Eric T. Harvill, Illiassou Hamidou Soumana, Amanda D. Caulfield, Monica Cartelle Gestal |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Bordetella pertussis Whooping Cough Epidemiology Infectious and parasitic diseases RC109-216 Targeted Nasopharyngeal Bordetella pertussis Inoculation in Mice Reveals Immune Evasion and Models Vaccine Limitations respiratory infections Mice Immune system Nasopharynx Animals Medicine Colonization Respiratory system bacteria asymptomatic infection Bordetella Infections Immune Evasion Pertussis Vaccine Lung biology business.industry Research vaccines biology.organism_classification medicine.disease Infectious Diseases medicine.anatomical_structure Upper respiratory tract infection upper respiratory tract infection Immunization Immunology business Respiratory tract |
| Zdroj: | Emerging Infectious Diseases Emerging Infectious Diseases, Vol 27, Iss 8, Pp 2107-2116 (2021) |
| ISSN: | 1080-6059 1080-6040 |
| DOI: | 10.3201/eid2708.203566 |
| Popis: | Conventional pertussis animal models deliver hundreds of thousands of Bordetella pertussis bacteria deep into the lungs, rapidly inducing severe pneumonic pathology and a robust immune response. However, human infections usually begin with colonization and growth in the upper respiratory tract. We inoculated only the nasopharynx of mice to explore the course of infection in a more natural exposure model. Nasopharyngeal colonization resulted in robust growth in the upper respiratory tract but elicited little immune response, enabling prolonged and persistent infection. Immunization with human acellular pertussis vaccine, which prevents severe lung infections in the conventional pneumonic infection model, had little effect on nasopharyngeal colonization. Our infection model revealed that B. pertussis can efficiently colonize the mouse nasopharynx, grow and spread within and between respiratory organs, evade robust host immunity, and persist for months. This experimental approach can measure aspects of the infection processes not observed in the conventional pneumonic infection model. |
| Databáze: | OpenAIRE |
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