Effects of Polygonum cuspidatum on AMPK-FOXO3α Signaling Pathway in Rat Model of Uric Acid-Induced Renal Damage
| Autor: | Fengxian Meng, Yuxi He, Hong-hong Zhang, Zhengju Zhang, Xiang-wei Bu, Jie Wang, Jian-ping Zhang, Xiaoxu Zhang, Dali Wang, Wei-guo Ma |
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| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_specialty 0211 other engineering and technologies Hyperuricemia 02 engineering and technology AMP-Activated Protein Kinases 030226 pharmacology & pharmacy Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot AMP-activated protein kinase Fallopia japonica Internal medicine 021105 building & construction medicine Animals Pharmacology (medical) Protein kinase A Chemokine CCL2 Messenger RNA medicine.diagnostic_test biology Plant Extracts Chemistry Forkhead Box Protein O3 AMPK Biological activity General Medicine Rats Uric Acid Reverse transcription polymerase chain reaction Disease Models Animal Endocrinology Complementary and alternative medicine biology.protein Uric acid Kidney Diseases Signal Transduction |
| Zdroj: | Chinese Journal of Integrative Medicine. 25:182-189 |
| ISSN: | 1993-0402 1672-0415 |
| Popis: | To observe the effects of Chinese medicine (CM) Polygonum cuspidatum (PC) on adenosine 5′-monophosphate-activated protein kinase (AMPK), forkhead box O3α (FOXO3α), Toll-like receptor-4 (TLR4), NACHT, LRR and PYD domains-containing protein 3 (NLRP3), and monocyte chemoattractant protein-1 (MCP-1) expression in a rat model of uric acid-induced renal damage and to determine the molecular mechanism. A rat model of uric acid-induced renal damage was established, and rats were randomly divided into a model group, a positive drug group, and high-, medium-, and low-dose PC groups (n=12 per group). A normal group (n=6) was used as the control. Rats in the normal and model groups were administered distilled water (10 mL•kg–1) by intragastric infusion. Rats in the positive drug group and the high-, medium-, and low-dose PC groups were administered allopurinol (23.33 mg•kg–1), and 7.46, 3.73, or 1.87 g•kg–1•d–1 PC by intragastric infusion, respectively for 6 to 8 weeks. After the intervention, reverse transcription polymerase chain reaction, Western blot, enzyme linked immunosorbent assay, and immunohistochemistry were used to detect AMPK, FOXO3α, TLR4, NLRP3, and MCP-1 mRNA and protein levels in renal tissue or serum. Compared with the normal group, the mRNA transcription levels of AMPK and FOXO3α in the model group were significantly down-regulated, and protein levels of AMPKα1, pAMPKα1 and FOXO3α were significantly down-regulated at the 6th and 8th weeks (P |
| Databáze: | OpenAIRE |
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