Correction to: Foy KC, Miller MJ, Moldovan N, Carson III WE, Kaumaya PTP. Combined vaccination with HER-2 peptide followed by therapy with VEGF peptide mimics exerts effective anti-tumor and anti-angiogenic effects in vitro and in vivo. OncoImmunology 2012; 1:1048–1060
| Autor: | Pravin P. T. Kaumaya, Kevin Chu Foy, Megan J. Miller, William E. Carson, Nicanor I. Moldovan |
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| Rok vydání: | 2013 |
| Předmět: |
Antitumor activity
chemistry.chemical_classification peptides/epitopes biology business.industry VEGF receptors Immunology Anti angiogenic Peptide peptidomimetic In vitro Vaccination angiogenesis Oncology chemistry In vivo peptides biology.protein Cancer research antibodies Immunology and Allergy Medicine business Research Paper |
| Zdroj: | Oncoimmunology |
| ISSN: | 2162-402X |
| DOI: | 10.4161/onci.23914 |
| Popis: | Overexpression of HER-2 and VEGF plays a key role in the development and metastasis of several human cancers. Many FDA-approved therapies targeting both HER-2 (Trastuzumab, Herceptin) and VEGF (Bevacizumab, Avastin) are expensive, have unacceptable toxicities and are often associated with the development of resistance. Here, we evaluate the dual antitumor effects of combining designed particular HER-2 peptide vaccine with VEGF peptide mimics. In vitro, HER-2 phosphorylation and antibody-dependent cellular toxicity were used to validate whether combining HER-2- and VEGF-targeting therapies would be effective. Moreover, a two-pronged approach was tested in vivo: (1) active immunotherapy with conformational HER-2 B-cell epitope vaccines and (2) anti-angiogenic therapy with a peptide structured to mimic VEGF. A transplantable BALB/c mouse model challenged with TUBO cells was used to test the effects of the HER-2 peptide vaccine combined with VEGF peptide mimics. Tumor sections after treatment were stained for blood vessel density and actively dividing cells. Our results show that immunization with an HER-2 peptide epitope elicits high affinity HER-2 native antibodies that are effective in inhibiting tumor growth in vivo, an effect that is enhanced by VEGF peptide mimics. We demonstrate that the combination of HER-2 and VEGF peptides induces potent anti-tumor and anti-angiogenic responses. |
| Databáze: | OpenAIRE |
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