Role of Endoplasmic Reticulum Stress in Nano-Selenium Alleviating Prehierarchical Follicular Atresia Induced by Mercury in Laying Hens
| Autor: | Yan Ma, Yizhen Shi, Yuqin Wang, Qiujue Wu, Binyao Cheng, Yumeng Li, Zhuosi Wang, Xiaoying Chai, Ao Ren, Gan Li |
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| Rok vydání: | 2021 |
| Předmět: |
Estradiol
Caspase 3 Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Follicular Atresia General Medicine Mercury Luteinizing Hormone Protein Serine-Threonine Kinases Endoplasmic Reticulum Stress TNF Receptor-Associated Factor 2 Biochemistry Caspase 9 Inorganic Chemistry Selenium Endoribonucleases Animals Female Follicle Stimulating Hormone Chickens Progesterone bcl-2-Associated X Protein |
| Zdroj: | Biological trace element research. 200(12) |
| ISSN: | 1559-0720 |
| Popis: | This study investigated that the effect of nano-selenium (nano-Se) addition preventing prehierarchical follicular atresia induced by mercury (Hg) exposure in laying hens. Furthermore, endoplasmic reticulum (ER) stress pathway was explored to reveal the protective mechanism of nano-Se in vitro. The results revealed that Hg could significantly reduce laying performance (P 0.05) and egg quality (P 0.05), whereas nano-Se addition partially reversed the reductions. Besides, Hg significantly induced the deposition of Hg in prehierarchical follicles (P 0.05) and prehierarchical follicular atresia (P 0.05), whereas nano-Se addition could alleviate these toxicities in vitro. In addition, Hg exposure could significantly reduce cell viability (P 0.05) and induce pyknotic nucleus in prehierarchical granulosa cells, while nano-Se addition reversed these effects. The levels of follicle-stimulating hormone (P 0.05), luteinizing hormone (P 0.05), progesterone (P 0.05), and estradiol (P 0.05) were significantly decreased after Hg exposure in vitro. However, nano-Se addition reversed the decreases of sex hormone levels. Furthermore, Hg exposure significantly increased the gene expressions of CHOP (P 0.05), PERK (P 0.05), ATF4 (P 0.05), ATF6 (P 0.05), ASK1 (P 0.05), IRE1α (P 0.05), TRAF2 (P 0.05), caspase-9 (P 0.05), caspase-3 (P 0.05), and Bax/Bcl-2 (P 0.05), whereas nano-Se addition reversed these increases of gene expressions in vitro. In summary, this study provides that Hg can induce prehierarchical follicular atresia, whereas nano-Se addition can ameliorate it, and elucidates an important role of ER stress in nano-Se alleviating prehierarchical follicular atresia induced by Hg in laying hens. |
| Databáze: | OpenAIRE |
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