The mature virion of ectromelia virus, a pathogenic poxvirus, is capable of intrahepatic spread and can serve as a target for delayed therapy
| Autor: | Luis J. Sigal, J. Charles Whitbeck, Felicia Roscoe, Gary H. Cohen, Xueying Ma, Roselyn J. Eisenberg, Ren-Huan Xu |
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| Rok vydání: | 2013 |
| Předmět: |
Ectromelia virus
viruses Immunology Mice SCID Antibodies Viral Microbiology Virus chemistry.chemical_compound Mice Cytosol Viral envelope Viral entry Virology Animals Ectromelia Infectious Pathogen Mice Inbred BALB C biology Virion Antibodies Monoclonal biology.organism_classification Mice Inbred C57BL chemistry Liver Insect Science Immunoglobulin G Monkeypox virus Pathogenesis and Immunity Variola virus Vaccinia |
| Zdroj: | Journal of virology. 87(12) |
| ISSN: | 1098-5514 |
| Popis: | Orthopoxviruses (OPVs), which include the agent of smallpox (variola virus), the zoonotic monkeypox virus, the vaccine and zoonotic species vaccinia virus, and the mouse pathogen ectromelia virus (ECTV), form two types of infectious viral particles: the mature virus (MV), which is cytosolic, and the enveloped virus (EV), which is extracellular. It is believed that MVs are required for viral entry into the host, while EVs are responsible for spread within the host. Following footpad infection of susceptible mice, ECTV spreads lymphohematogenously, entering the liver at 3 to 4 days postinfection (dpi). Afterwards, ECTV spreads intrahepatically, killing the host. We found that antibodies to an MV protein were highly effective at curing mice from ECTV infection when administered after the virus reached the liver. Moreover, a mutant ECTV that does not make EV was able to spread intrahepatically and kill immunodeficient mice. Together, these findings indicate that MVs are sufficient for the spread of ECTV within the liver and could have implications regarding the pathogenesis of other OPVs, the treatment of emerging OPV infections, as well as strategies for preparedness in case of accidental or intentional release of pathogenic OPVs. |
| Databáze: | OpenAIRE |
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