Effect of Sample Collection (Manual Expression vs. Pumping) and Skimming on the Microbial Profile of Human Milk Using Culture Techniques and Metataxonomic Analysis
| Autor: | Maria de los Angeles Checa, Claudio Alba, Leónides Fernández, Juan M. Rodríguez, Maricela Rodríguez-Cruz, Marina Aparicio |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) manual collection food.ingredient Sample (material) 030106 microbiology Infant health Biology Microbiology Article 03 medical and health sciences food fluids and secretions Virology Lactation Skimmed milk medicine microbiota Food science lcsh:QH301-705.5 metataxonomic analysis food and beverages human milk skimming culture techniques pumps Manual expression Whole milk 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Sample collection |
| Zdroj: | Microorganisms Volume 8 Issue 9 Microorganisms, Vol 8, Iss 1278, p 1278 (2020) |
| ISSN: | 2076-2607 |
| Popis: | Human milk microbiota is a unique bacterial community playing a relevant role in infant health, but its composition depends on different factors (woman health, lactation stage, and geographical lactation). However, information is lacking regarding some other factors that may affect the bacterial community of human milk. In this study we aimed to study the impact of the sample collection method and the skimming procedure using culture-dependent and culture-independent techniques to study the human milk microbial profile. One set of milk samples was provided by women (n = 10) in two consecutive days half of the samples were collected the first day by manual expression and the other half on the second day by pumping. The rest of the participants (n = 17) provided milk samples that were fractionated by centrifugation the bacterial profiles of whole milk and skimmed milk were compared by culture techniques in 10 milk samples, while those of whole milk, fat and skimmed milk were subjected to metataxonomic analysis in seven samples. Globally, the results obtained revealed high interindividual variability but that neither the use of single-use sterile devices to collect the sample nor the skimming procedure have a significant impact of the microbial profile of human samples. |
| Databáze: | OpenAIRE |
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