Transfer of cow's milk beta-lactoglobulin to human serum after a milk load: a pilot study
| Autor: | Julie A. Lovegrove, D L Osman, J B Morgan, S M Hampton |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Male medicine.medical_specialty Enzyme-Linked Immunosorbent Assay Pilot Projects Lactoglobulins Cross Reactions Immunoglobulin E Sensitivity and Specificity Intestinal absorption Immunoglobulin G Antigen Internal medicine medicine Animals Humans Beta-lactoglobulin biology Gastroenterology food and beverages Milk Postprandial Endocrinology Intestinal Absorption biology.protein Cattle Female Antibody Research Article |
| Zdroj: | Gut. 34:203-207 |
| ISSN: | 0017-5749 |
| DOI: | 10.1136/gut.34.2.203 |
| Popis: | A sensitive and specific enzyme linked immunosorbent assay (ELISA) was developed for the quantification of cow's milk beta-lactoglobulin in human serum. The assay had a sensitivity of 80 pg/ml (2 standard deviations from zero) and did not cross react significantly with any other tested milk proteins. The absorption of cow's milk beta-lactoglobulin and beta-lactoglobulin immunoglobulin G antibody levels after a milk load was investigated in eight healthy non-allergic individuals. There was a significant variation in the circulating beta-lactoglobulin antigen levels during the six hour period after feeding (p < 0.01) for the combined data. A biphasic absorption pattern was seen in all subjects except one subject who had undetectable levels. No significant variation with time was observed for the beta-lactoglobulin IgG antibody levels and no correlation was found between the beta-lactoglobulin antigen levels and the beta-lactoglobulin IgG antibody levels. This pilot study offered information on the quantity of immunogenic cows' milk protein transferred to the blood after a milk load in non-allergic healthy adults and confirmed a biphasic profile of antigen transfer. The sensitivity of the assay used has shown that transfer of food antigen does occur in the majority of healthy nonallergic subjects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|