Colocalization of synaptic GABAC-receptors with GABAA-receptors and glycine-receptors in the rodent central nervous system
| Autor: | Renata Frazão, Maria Inês Nogueira, Heinz Wässle |
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| Rok vydání: | 2007 |
| Předmět: |
Nervous system
Superior Colliculi Histology Pathology and Forensic Medicine GABAA-rho receptor Mice Receptors Glycine Receptors GABA Postsynaptic potential medicine Animals Rats Wistar Receptor Glycine receptor GABAA receptor Chemistry Colocalization Cell Biology Receptors GABA-A Immunohistochemistry Inferior Colliculi Rats Cell biology Mice Inbred C57BL medicine.anatomical_structure Spinal Cord nervous system Neuroscience Brain Stem Ionotropic effect |
| Zdroj: | Cell and Tissue Research. 330:1-15 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-007-0446-y |
| Popis: | Fast inhibition in the nervous system is preferentially mediated by GABA- and glycine-receptors. Two types of ionotropic GABA-receptor, the GABA(A)-receptor and GABA(C)-receptor, have been identified; they have specific molecular compositions, different sensitivities to GABA, different kinetics, and distinct pharmacological profiles. We have studied, by immunocytochemistry, the synaptic localization of glycine-, GABA(A)-, and GABA(C)-receptors in rodent retina, spinal cord, midbrain, and brain-stem. Antibodies specific for the alpha1 subunit of the glycine-receptor, the gamma2 subunit of the GABA(A)-receptor, and the rho subunits of the GABA(C)-receptor have been applied. Using double-immunolabeling, we have determined whether these receptors are expressed at the same postsynaptic sites. In the retina, no such colocalization was observed. However, in the spinal cord, we found the colocalization of glycine-receptors with GABA(A)- or GABA(C)-receptors and the colocalization of GABA(A)- and GABA(C)-receptors in approximately 25% of the synapses. In the midbrain and brain-stem, GABA(A)- and GABA(C)-receptors were colocalized in 10%-15% of the postsynaptic sites. We discuss the possible expression of heteromeric (hybrid) receptors assembled from GABA(A)- and GABA(C)-receptor subunits. Our results suggest that GABA(A)- and GABA(C)-receptors are colocalized in a minority of synapses of the central nervous system. |
| Databáze: | OpenAIRE |
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