Self-Assembled Magnetic Bead Biosensor for Measuring Bacterial Growth and Antimicrobial Susceptibility Testing

Autor: Raoul Kopelman, Sima Mofakham, Paivo Kinnunen, Irene Sinn, Theodore Albertson, Remy Elbez, Brandon H. McNaughton, Alan W. Hunt, Duane W. Newton
Rok vydání: 2012
Předmět:
Zdroj: Small. 8:2477-2482
ISSN: 1613-6810
Popis: As indicated by leading healthcare agencies like the Centers for Disease Control and Prevention (CDC) and the World Health Organization (WHO), the rising antibiotic resistance of bacteria is one of the major concerns of modern healthcare, and the development of novel growth-based rapid diagnostics is a key in addressing this problem.[1,2] A common growth-based test used to help guide therapy is the antimicrobial susceptibility test (AST). Faster AST testing can lead to reduced inappropriate antibiotic use, decreased health care costs, and reduced prevalence of antimicrobial resistance.[3,4] To address this problem our group has been developing asynchronous magnetic bead rotation (AMBR) biosensors that have been used to detect the growth of individual bacterial cells.[5] Here, we introduce a radically new type of AMBR sensor that detects bacterial growth-based on the rotation of a cluster of magnetic microparticles formed through self-assembly. Using these self-assembled AMBR biosensors, the minimum inhibitory concentration (MIC) value of a uropathogenic Escherichia coli isolate was measured. While detection of the presence of pathogens is a widely covered topic in research and assay development, here we focus on demonstrating that AMBR can be used to perform AST testing. A new method for detecting the rotation of AMBR biosensors is also described, which hass enabled validation of a multi-well prototype for rapid observation of bacterial growth.
Databáze: OpenAIRE
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