In vitro DNA synthesis as indicator of mammary epithelial cell division: [14C]thymidine uptake versus flow cytometry cell cycle analysis
| Autor: | J. P. Van Zyl, J. E. Keys, H. M. Farrell |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Cell division Population Adipose tissue Biology Epithelium Flow cytometry chemistry.chemical_compound Mammary Glands Animal Internal medicine medicine Animals Carbon Radioisotopes education education.field_of_study medicine.diagnostic_test DNA synthesis Cell Cycle Epithelial Cells DNA Cell Biology General Medicine Cell cycle Flow Cytometry Molecular biology Endocrinology Adipose Tissue Liver chemistry Cell culture Cattle Female sense organs Thymidine Cell Division Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 30:50-55 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/bf02631418 |
| Popis: | Mammary and adipose explants from eight mid-lactation Holstein cows were co-cultured for 24 h in the presence or absence of liver explants, 1 microgram/ml pituitary bovine somatotrophin, or 100 ng/ml insulinlike growth factor-I. Liver explants in the media significantly depressed DNA and protein synthesis by mammary tissue as measured by [14C]-thymidine and amino acid incorporation. As measured by flow cytometry, the concentration of DNA in the G0G1 and G2M cells and the percentage of cells in the G0G1 population of mammary tissue was also significantly depressed by liver tissue. Changes in the percentage of cells in the S and G2M phases were not significant. Insulinlike growth factor-I in the presence of liver explants depressed protein synthesis, thymidine incorporation, and the concentration of DNA in the G0G1 and G2M cells compared to control but did not affect the percentage of cells in the G0G1, S, or G2M phases. Previously it was assumed that changes in [14C]thymidine incorporation indicated that changes in cell division were occurring. Flow cytometry revealed that changes in DNA content of mammary cells as a result of liver or hormonal stimulation were not due to changes in cell division. Indications are that differences in cellular DNA content result from changes in the rate of amplification of individual genes responsible for milk protein synthesis. |
| Databáze: | OpenAIRE |
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