Immunological milieu in the peritoneal cavity at laparotomy for gastric cancer
| Autor: | Seiya Susumu, Ken Taniguchi, Akira Yoneda, Takashi Kanematsu, Mitsutoshi Matsuo, Yasuhiro Nagata, Yoshitsugu Tajima, Susumu Eguchi, Shinichiro Ito |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Th2 response Regulatory T cell medicine.medical_treatment T-Lymphocytes Gastroenterology Peritoneal cavity Th2 Cells Gastrectomy Stomach Neoplasms Internal medicine Laparotomy medicine Animals Humans Peritoneal Lavage Lymphocytes Cells Cultured Aged business.industry digestive oral and skin physiology Cancer General Medicine Advanced gastric cancer Middle Aged medicine.disease Flow Cytometry digestive system diseases Coculture Techniques Surgery medicine.anatomical_structure Leukocytes Mononuclear Cytokines Original Article Female business Gastric cancer |
| Zdroj: | World Journal of Gastroenterology. 18(13):1470-1478 |
| ISSN: | 1007-9327 |
| Popis: | AIM: To investigate the immunological repertoire in the peritoneal cavity of gastric cancer patients. METHODS: The peritoneal cavity is a compartment in which immunological host-tumor interactions can occur. However, the role of lymphocytes in the peritoneal cavity of gastric cancer patients is unclear. We observed 64 patients who underwent gastrectomy for gastric cancer and 11 patients who underwent laparoscopic cholecystectomy for gallstones and acted as controls. Lymphocytes isolated from both peripheral blood and peritoneal lavage were analyzed for surface markers of lymphocytes and their cytokine production by flow cytometry. CD4 +CD25 high T cells isolated from the patient's peripheral blood were co-cultivated for 4 d with the intra-peritoneal lymphocytes, and a cytokine assay was performed. RESULTS: At gastrectomy, CCR7 - CD45RA - CD8 + effector memory T cells were observed in the peritoneal cavity. The frequency of CD4 + CD25 high T cells in both the peripheral blood and peritoneal cavity was elevated in patients at advanced stage [control vs stage IV in the peripheral blood: 6.89 (3.39-10.4) vs 15.34 (11.37-19.31), P < 0.05, control vs stage IV in the peritoneal cavity: 8.65 (5.28-12.0) vs 19.56 (14.81-24.32), P < 0.05]. On the other hand, the suppression was restored with CD4 + CD25 high T cells from their own peripheral blood. This study is the first to analyze lymphocyte and cytokine production in the peritoneal cavity in patients with gastric cancer. Immune regulation at advanced stage is reversible at the point of gastrectomy. CONCLUSION: The immunological milieu in the peritoneal cavity of patients with advanced gastric cancer elicited a Th2 response even at gastrectomy, but this response was reversible. World Journal of Gastroenterology, 18(13), pp.1470-1478; 2012 |
| Databáze: | OpenAIRE |
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