Specific neuronal subpopulations in the rat basolateral amygdala express high levels of nonphosphorylated neurofilaments
Autor: | Franco Mascagni, Alexander J. McDonald |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine Neurofilament education Intermediate Filaments Neuropeptide Biology Amygdala Article Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine mental disorders medicine Animals Phosphorylation Neurons Immunoperoxidase Basolateral Nuclear Complex Pyramidal Cells General Neuroscience fungi Molecular biology Retrograde tracing Rats 030104 developmental biology medicine.anatomical_structure nervous system Cerebral cortex Nucleus 030217 neurology & neurosurgery Basolateral amygdala |
Zdroj: | J Comp Neurol |
ISSN: | 1096-9861 0021-9967 |
Popis: | Cortical pyramidal neurons (PNs) containing non-phosphorylated neurofilaments (NNFs) localized with the SMI-32 monoclonal antibody have been shown to be especially vulnerable to degeneration in Alzheimer's disease (AD). The present investigation is the first to study the expression of SMI-32+ NNFs in neurons of the basolateral nuclear complex of the amygdala (BNC), which contains cortex-like PNs and nonpyramidal neurons (NPNs). We observed that PNs in the rat basolateral nucleus (BL), but not in the lateral (LAT) or basomedial (BM) nuclei, have significant levels of SMI-32-ir in their somata with antibody diluents that did not contain Triton X-100, but staining in these cells was greatly attenuated when the antibody diluent contained 0.3% Triton. Using Triton-containing diluents we found that all SMI-32+ neurons in all three of the BNC nuclei were NPNs. Using a dual-labeling immunoperoxidase technique we demonstrated that most of these SMI-32+ NPNs were parvalbumin-positive (PV+) or somatostatin-positive NPNs, but not vasoactive intestinal peptide-positive or neuropeptide Y-positive NPNs. Using a technique that combines retrograde tracing with SMI-32 immunohistochemistry using intermediate levels of Triton in the diluent we found that all BNC neurons projecting to the mediodorsal thalamic nucleus (MD) were large NPNs, and most were SMI-32+. In contrast, BNC neurons projecting to the ventral striatum or cerebral cortex were PNs that expressed low levels of SMI-32 immunoreactivity (SMI-32-ir) in the BL, and no SMI-32-ir in the LAT or BM. These data suggest that the main neuronal subpopulations in the BNC that degenerate in AD may be PV+ and MD-projecting NPNs. This article is protected by copyright. All rights reserved. |
Databáze: | OpenAIRE |
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