Effect of the Endosomal Acidification on Small Ion Transport Through the Anthrax Toxin PA63 Channel
| Autor: | Goli Yamini, Antonio Alcaraz, Nnanya Kalu, Clare Kenney, Ekaterina M. Nestorovich, Vicente M. Aguilella, Laura Lucas, Sanaz Momben Abolfath |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Endosome media_common.quotation_subject Anthrax toxin Multi-state conductance Bacterial Toxins Access resistance Biophysics Endosomes Biochemistry Article Ion Channels Cell Line 03 medical and health sciences chemistry.chemical_compound Anthrax toxin PA Structural Biology Genetics Animals Humans Internalization Molecular Biology Ion channel Ion transporter media_common Antigens Bacterial Ion Transport biology Chemistry Conductance Cell Biology Hydrogen-Ion Concentration biology.organism_classification Bacillus anthracis Current fluctuations 030104 developmental biology Channel (broadcasting) Ethylene glycol Endosomal acidification |
| Zdroj: | Repositori Universitat Jaume I Universitat Jaume I |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2017.11.3056 |
| Popis: | Tight regulation of pH is critical for the structure and function of cells and organelles. The pH environment changes dramatically along the endocytic pathway, an internalization transport process that is 'hijacked' by many intracellularly active bacterial exotoxins, including the anthrax toxin. Here, we investigate the role of pH on single-channel properties of the anthrax toxin protective antigen (PA63 ). Using conductance and current noise analysis, blocker binding, ion selectivity, and poly(ethylene glycol) partitioning measurements, we show that the channel exists in two different open states ('maximum' and 'main') at pH ≥ 5.5, while only a maximum conductance state is detected at pH |
| Databáze: | OpenAIRE |
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