Vitamins (A&D) and Isoprenoid (Chenodeoxycholic acid) molecules are accompanied by Th1 immunostimulatory response and therapeutic cure in vivo: possible antileishmanial drugs
| Autor: | Mohan Lal Dubey, Ramesh Kandimalla, Venkateswara R Gogulamudi, Deepak Kaul, Donfack Jean Hubert, Rakesh Sehgal |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Combination therapy medicine.medical_treatment Antiprotozoal Agents Retinoic acid lcsh:Medicine Pharmacology Chenodeoxycholic Acid Article Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Adjuvants Immunologic Immunity In vivo Target identification Chenodeoxycholic acid Animals Medicine lcsh:Science Vitamin A Cholecalciferol Mice Inbred BALB C Multidisciplinary business.industry lcsh:R Immunosuppression Th1 Cells medicine.disease 030104 developmental biology Visceral leishmaniasis chemistry Cytokines Leishmaniasis Visceral Infectious diseases lcsh:Q business 030217 neurology & neurosurgery Leishmania donovani |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Investigation of immune modulatory anti-leishmanial molecules is now being strongly encouraged to overcome the immunosuppression manifested during visceral leishmaniasis (VL), resistance, toxicity and high cost associated with conventional therapeutics. In the present study, we explored the protective efficacy of vitamin D3, retinoic acid and isoprenoid chenodeoxycholic acid (CDCA) combinations against L. donovani infected BALB/c mice. We also probed the immune modulatory response (Th1 & Th2 cytokines) and infection dynamics following experimental infections with drug treated animals. Our results indicate that Vit.D3/RA and CDCA/RA combination treatment led to significant inhibition of parasite load on days 21 and 28 post treatment. Furthermore, there was a marked inhibition of Th2 type immune responses in IL-4, IL-5 and polarization of Th1 biased immunity along with upregulation of IL-1, IFN-γ, and TNF-α levels on day 28 post treatment. In addition, mice treated with Vit.D3/RA and CDCA/RA demonstrates here that splenic histological recovery against the virulent challenge of L. donovani by day 28 was comparable to control group. The conclusions derived from this study suggests that a combination of vitamin A, D3 and isoprenoids may have a potential immunomodulatory therapeutic role against leishmaniasis. |
| Databáze: | OpenAIRE |
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