Transient Expression of Homologous Hairpin RNA Interferes with Broad bean wilt virus 2 Infection in Nicotiana benthamiana
| Autor: | Soon Bae Kwon, Ju-Yeon Yoon, Peter Palukaitis, Ki Hyun Ryu, Seung-Kook Choi, Jin Woo Park, Gug Sun Choi |
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| Rok vydání: | 2012 |
| Předmět: |
Small interfering RNA
Agroinfiltration Resistance RT-PCR Nicotiana benthamiana Plant Science Biology Biochemistry Broad bean wilt virus 2 Small hairpin RNA Broad bean wilt virus Secoviridae lcsh:Agriculture (General) Comovirinae Molecular Biology Transient fungi food and beverages RNA Small-interfering RNA biology.organism_classification lcsh:S1-972 Virology Agronomy and Crop Science Biotechnology |
| Zdroj: | Research in Plant Disease, Vol 18, Iss 4, Pp 268-276 (2012) |
| ISSN: | 1598-2262 |
| DOI: | 10.5423/rpd.2012.18.4.268 |
| Popis: | Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea(Received on October 30, 2012; Revised on November 15, 2012; Accepted on November 26, 2012)Broad bean wilt virus 2 (BBWV2), genus Fabavirus, subfamily Comovirinae, family Secoviridae, causesdamage in many economically important horticultural and ornamental crops. Sequence alignments showedseveral conserved sequences in 5' non-coding regions (5' NCRs) of RNA 1 and RNA 2 in all BBWV2 strainscharacterized so far. Based on this observation, we generated a hpRNA construct (pIR-BBWV2) harboringan inverted repeat containing a 210 bp cDNA fragment homologous to 5' NCR portion of BBWV2 RNA 1 toinvestigate the silencing potential for its ability to interfere with a rapidly replicating BBWV2. Agrobacterium-mediated transient expression of the IR-BBWV2 had a detrimental effect on BBWV2 infection, showing nodistinct symptoms in non-inoculated leaves of the agroinfiltrated Nicotiana benthamiana plants. BBWV2genomic RNAs were not detected by RT-PCR from tissues of both the inoculated leaves and upper leaves ofthe agroinfiltrated plants. Accumulation of virus-derived small interfering RNAs was detected in theinoculated leaf tissues of N. benthamiana plants elicited by transient expression of IR-BBWV2 indicating thatRNA silencing is responsible for the resistance to BBWV2.Keywords : Agroinfiltration, Broad bean wilt virus 2, Resistance, RT-PCR, Small-interfering RNA, Transientexpression |
| Databáze: | OpenAIRE |
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