Development, physicochemical characterization and cytotoxicity of selenium nanoparticles stabilized by beta-lactoglobulin
| Autor: | Zi Teng, Zhiyuan Lou, Qin Wang, Qing-Zhu Zeng, Yang Yuan, Seong-Ho Lee, Jinglin Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_element Infrared spectroscopy Nanoparticle 02 engineering and technology Lactoglobulins Biochemistry Fluorescence 03 medical and health sciences Selenium Dynamic light scattering Structural Biology Nephelometry and Turbidimetry Spectrophotometry Spectroscopy Fourier Transform Infrared medicine Organic chemistry Humans Fourier transform infrared spectroscopy Particle Size Molecular Biology medicine.diagnostic_test Cell Death General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Ascorbic acid HCT116 Cells Solutions 030104 developmental biology chemistry Nanoparticles Particle size 0210 nano-technology Nuclear chemistry |
| Zdroj: | International journal of biological macromolecules. 107 |
| ISSN: | 1879-0003 |
| Popis: | Novel Selenium nanoparticles (SeNPs) were developed using beta-lactoglobulin (Blg) as a stabilizer in redox systems of selenite and ascorbic acid in this study. Particle size, morphology, stability, and in vitro biological activity of synthesized Blg stabilized selenium nanoparticles (Blg-SeNPs) were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), ultraviolet-visible spectrophotometry (UV/Vis), and cell toxicity assays, respectively. Stabilizing mechanisms of Blg-SeNPs were investigated by Fourier-transform infrared spectroscopy (FTIR) and protein fluorescence probe. The results revealed that the Blg-SeNPs were spherical with mean particle size of 36.8±4.1nm. They were stable in acidic or neutral to basic solutions (pH 2.5-3.5 or 6.5-8.5) at 4°C for 30days as a result of electrostatic repulsions. FTIR results showed that functional groups of NH2 and OH on Blg molecules were responsible for binding with SeNPs. Furthermore, decreases in protein surface hydrophobicity indicated that possible binding happened between Se and the hydrophobic domains of Blg. The cell toxicity of Blg-SeNPs was significantly lower than that of sodium selenite on both cancerous and non-cancerous cells. This study provides a facile and green method for chemically synthesizing stable SeNPs which are suitable for further evaluation in medicinal applications. |
| Databáze: | OpenAIRE |
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