Generation of patient-specific induced pluripotent stem cells (ZZUSAHi001-A) derived from a familial atrial fibrillation patient carrying KCNA5 c.775GA mutation
| Autor: | Haiyan Xiong, Shaohui Niu, Lihua Zhang, Yuan Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Biology General Medicine Germ layer Biology medicine.disease Peripheral blood mononuclear cell Viral vector 03 medical and health sciences 030104 developmental biology 0302 clinical medicine lcsh:Biology (General) SOX2 KLF4 embryonic structures Cancer research medicine Induced pluripotent stem cell lcsh:QH301-705.5 Reprogramming 030217 neurology & neurosurgery Familial atrial fibrillation Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 47, Iss, Pp 101915-(2020) |
| ISSN: | 1876-7753 |
| Popis: | Peripheral blood mononuclear cells (PBMCs) were isolated from a 57-year-old female who was clinically diagnosed as familial atrial fibrillation and were reprogrammed using non-integrative Sendai viral vectors containing reprogramming factors OCT4, SOX2, KLF4 and cMYC. The generated human induced pluripotent stem cell (hiPSC) line, ZZUSAHi001-A, exhibited a normal karyotype, showed robust expression of pluripotency markers and differentiated towards three germ layers in vivo. |
| Databáze: | OpenAIRE |
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