In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever
| Autor: | Vijayakumar Subramaniyan, Ramesh Venkatachalam, Prabhu Srinivasan, Manogar Palani |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Serotype Dengue virus medicine.disease_cause General Biochemistry Genetics and Molecular Biology Epitope Dengue fever 03 medical and health sciences 0302 clinical medicine Antigen vaccine medicine 030212 general & internal medicine dengue serotypes biology dengue virus Reverse vaccinology General Medicine medicine.disease Virology 030104 developmental biology E-proteins biology.protein Peptide vaccine MHC I and II Original Article Antibody |
| Zdroj: | Journal of Biomedical Research |
| ISSN: | 1674-8301 |
| Popis: | Reverse vaccinology method was used to predict the monovalent peptide vaccine candidate to produce antibodies for therapeutic purpose and to predict tetravalent vaccine candidate to act as a common vaccine to cover all the fever dengue virus serotypes. Envelope (E)-proteins of DENV-1-4 serotypes were used for vaccine prediction using NCBI, Uniprot/Swissprot, Swiss-prot viewer, VaxiJen V2.0, TMHMM, BCPREDS, Propred-1, Propred and MHC Pred,. E-proteins of DENV-1-4 serotypes were identified as antigen from which T cell epitopes, through B cell epitopes, were predicted to act as peptide vaccine candidates. Each selected T cell epitope of E-protein was confirmed to act as vaccine and to induce complementary antibody against particular serotype of dengue virus. Chimeric tetravalent vaccine was formed by the conjugation of four vaccines, each from four dengue serotypes to act as a common vaccine candidate for all the four dengue serotypes. It can be justifiably concluded that the monovalent 9-mer T cell epitope for each DENV serotypes can be used to produce specific antibody agaomst dengue virus and a chimeric common tetravalent vaccine candidate to yield a comparative vaccine to cover any of the four dengue virus serotype. This vaccine is expected to act as highly immunogenic against preventing dengue fever. |
| Databáze: | OpenAIRE |
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