Human peripheral blood eosinophils produce and release interleukin-8 on stimulation with calcium ionophore
| Autor: | T T Hansel, Silvia Rihs, I. Jolanda M. De Vries, Francois Erard, C. Walker, Marco Franchini, Ruedi K. Braun, Kurt Blaser |
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| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
T-Lymphocytes Immunology Ionophore chemistry.chemical_element Gene Expression Calcium Biology In Vitro Techniques Polymerase Chain Reaction Internal medicine Cyclosporin a medicine Immunology and Allergy Macrophage Humans Interleukin 8 RNA Messenger Calcimycin Cells Cultured Interleukin-8 Interleukin Granulocyte-Macrophage Colony-Stimulating Factor Eosinophil Eosinophils medicine.anatomical_structure Endocrinology chemistry Leukocytes Mononuclear Tumor necrosis factor alpha Interleukin-3 Granulocytes |
| Zdroj: | European journal of immunology. 23(4) |
| ISSN: | 0014-2980 |
| Popis: | We present evidence that human blood eosinophils produce interleukin (IL)-8 when stimulated with calcium ionophore. Following in vitro culture of 99 % pure eosinophils with calcium ionophore, released IL-8 was detectable by enzymelinked immunosorbent assay in supernatants. Eosinophil IL-8 production was considerably greater than that of IL-3 or granulocyte macrophage colony-stimulating factor. Furthermore, eosinophil production of IL-8 in the presence of calcium ionophore could be inhibited with the immunomodulating agent cyclosporin A and the protein synthesis inhibitor cycloheximide. In addition, following stimulation of highly purified blood eosinophils with calcium ionophore, IL-8 mRNA was detectable after polymerase chain reaction amplification. In comparison with other cells on stimulation with calcium ionophore, eosinophils produce about half as much IL-8 as neutrophils but significantly more than purified T cells. In contrast to monocytes and neutrophils, IL-8 production was not inducible with IL-1 or tumor necrosis factor. Finally, following calcium ionophore stimulation blood eosinophils were shown to contain cytoplasmic IL-8 by employing a monoclonal antibody against IL-8 in conjunction with immuno-histochemistry. These observations demonstrate that eosinophils are capable of IL-8 production and release, which may contribute to defense against parasites and to the pathophysiology of allergic and asthmatic disease. |
| Databáze: | OpenAIRE |
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