The carboxy-terminal peptides of 46 kDa and 300 kDa mannose 6-phosphate receptors share partial sequence homology and contain information for sorting in the early endosomal pathway
| Autor: | C. Boker, Annette Hille-Rehfeld, K von Figura |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Endosome
media_common.quotation_subject Molecular Sequence Data Endocytic cycle Mannose Peptide Endosomes Mannose 6-phosphate Biology Receptor IGF Type 2 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Humans Amino Acid Sequence Receptor Internalization Cells Cultured 030304 developmental biology media_common chemistry.chemical_classification 0303 health sciences Sequence Homology Amino Acid Cell Biology Fibroblasts Biochemistry chemistry Sequence Analysis 030217 neurology & neurosurgery Signal Transduction Cation-dependent mannose-6-phosphate receptor |
| Zdroj: | Journal of Cell Science. 110:1023-1032 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.110.8.1023 |
| Popis: | Recycling of mannose 6-phosphate receptors was investigated by microinjection of F(ab) fragments against their carboxy-terminal peptides (residues 54–67 or 150–164 of the cytoplasmic domain of 46 kDa and 300 kDa mannose 6-phosphate receptor, respectively). For each receptor, masking the carboxy-terminal peptide by the corresponding F(ab) fragments resulted in complete depletion of the intracellular pool. Redistributed 300 kDa mannose 6-phosphate receptor was shown to accumulate at the plasma membrane and to internalize anti-ectodomain antibodies. Internalization of anti-ectodomain antibodies was also observed for redistributed 46 kDa mannose 6-phosphate receptor. Semiquantitative analysis suggested that for both redistributed receptors the amount of intracellularly accumulated anti-ectodomain antibodies was reduced. In addition, downstream transport along the endosomal pathway was slowed down. These data suggest that sorting information for early steps in the endocytic pathway is contained within the carboxy-terminal peptides of mannose 6-phosphate receptors. |
| Databáze: | OpenAIRE |
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