Roles of 17 -Estradiol Involve Regulation of Reelin Expression and Synaptogenesis in the Dentate Gyrus
| Autor: | Lepu Zhou, Wiebke Wilkars, Roland A. Bender, Danny Paysen, Lars Fester, Gabriele M. Rune, Almut König, Jan-Simon Lanowski |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Cell Adhesion Molecules Neuronal Neurogenesis Cognitive Neuroscience Blotting Western Synaptogenesis Nerve Tissue Proteins In situ hybridization Hippocampal formation Hippocampus Synapse Mice Cellular and Molecular Neuroscience Organ Culture Techniques Microscopy Electron Transmission Internal medicine medicine Animals Estrogen Receptor beta Reelin Rats Wistar Aromatase In Situ Hybridization Extracellular Matrix Proteins Microscopy Confocal Estradiol biology Dentate gyrus Serine Endopeptidases Estrogen Receptor alpha Gene Expression Regulation Developmental DAB1 Immunohistochemistry Rats Reelin Protein Endocrinology nervous system Dentate Gyrus Synapses biology.protein Female |
| Zdroj: | Cerebral Cortex. 20:2985-2995 |
| ISSN: | 1460-2199 1047-3211 |
| DOI: | 10.1093/cercor/bhq047 |
| Popis: | Studies on the role of 17β-estradiol (E2) in the hippocampus have mainly focused on CA1 and CA3 regions, whereas in dentate gyrus (DG), its role is largely unknown. Here, we examined potential functions of E2 in DG, particularly during development. Immunohistochemistry and in situ hybridization revealed abundance of estrogen receptor (ER)α, but not ERβ, expression in DG. Similar to CA1, analysis of synapse densities revealed a reduction in spine synapse number in DG molecular layer of immature rats and adult mice after inhibition of estradiol synthesis using letrozole. Interestingly, strong expression of ERα was found in Cajal-Retzius (CR) cells, which regulate neuronal migration and synaptogenesis via the extracellular matrix protein reelin. Immunoreactivity of aromatase, the final enzyme of estradiol synthesis, was strongest in mature granule cells. In hippocampal slice cultures, exogenous application of E2 caused an increase in reelin expression in CR cells, which was abolished after blockade of ERs using ICI182,780. Vice versa, inhibition of aromatase activity by letrozole resulted in reduced reelin expression, suggesting that E2 deriving from hippocampal sources contributes to the regulation of reelin as well as to the maintenance of spine synapses in DG. E2 further regulated Notch1, a signaling protein involved in neuronal differentiation. |
| Databáze: | OpenAIRE |
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