Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis
| Autor: | Shishir Venkatesh, Xianfeng Chen, Vellaisamy A. L. Roy, Vaithinathan Karthikeyan, Clement Manohar Arava, Spoorthy Pathikonda, Peter K.N. Yu, Saw Lin Oo, Terrence Chi-Kong Lau |
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| Rok vydání: | 2021 |
| Předmět: |
cancer early detection
Cost-Benefit Analysis Early detection 02 engineering and technology Gastric carcinoma 010402 general chemistry lcsh:Chemical technology 01 natural sciences Biochemistry Article Analytical Chemistry polycarbonate membrane Stomach Neoplasms Cancer screening RNA modification medicine Biomarkers Tumor Humans lcsh:TP1-1185 Antigens Tumor-Associated Carbohydrate Electrical and Electronic Engineering Instrumentation Oligo RNA Early Detection of Cancer Chemistry Carcinoma RNA Cancer 021001 nanoscience & nanotechnology medicine.disease Atomic and Molecular Physics and Optics 0104 chemical sciences Highly sensitive Carcinoembryonic Antigen Cancer antigen Cancer research cancer antigen 0210 nano-technology electrical current sensor |
| Zdroj: | Sensors, Vol 21, Iss 2639, p 2639 (2021) Oo, S L, Venkatesh, S, Karthikeyan, V, Arava, C M, Pathikonda, S, Yu, P K N, Lau, T C K, Chen, M & Roy, V A L 2021, ' Highly Sensitive and Cost-Effective Portable Sensor for Early Gastric Carcinoma Diagnosis ', Sensors, vol. 21, no. 8, 2639 . https://doi.org/10.3390/s21082639 Sensors (Basel, Switzerland) Sensors Volume 21 Issue 8 |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s21082639 |
| Popis: | Facile and efficient early detection of cancer is a major challenge in healthcare. Herein we developed a novel sensor made from a polycarbonate (PC) membrane with nanopores, followed by sequence-specific Oligo RNA modification for early gastric carcinoma diagnosis. In this design, the gastric cancer antigen CA72-4 is specifically conjugated to the Oligo RNA, thereby inhibiting the electrical current through the PC membrane in a concentration-dependent manner. The device can determine the concentration of cancer antigen CA72-4 in the range from 4 to 14 U/mL, possessing a sensitivity of 7.029 µAU−1mLcm−2 with a linear regression (R2) of 0.965 and a lower detection limit of 4 U/mL. This device has integrated advantages including high specificity and sensitivity and being simple, portable, and cost effective, which collectively enables a giant leap for cancer screening technologies towards clinical use. This is the first report to use RNA aptamers to detect CA72-4 for gastric carcinoma diagnosis. |
| Databáze: | OpenAIRE |
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