Melanoma-initiating cells exploit M2 macrophage TGFβ and arginase pathway for survival and proliferation

Autor: Jean-Pierre Abastado, Veronique Angeli, Sandra Hubert, Luke Barron, Masashi Kato, Muly Tham, Jo Keeble, Xiaojie Wang, Armelle Prévost-Blondel, Kar Wai Tan, Nguan Soon Tan
Přispěvatelé: Singapore Immunology Network (SIgN), Biomedical Sciences Institute (BMSI), Department of Clinical Research, Singapore General Hospital, National Institute of Allergy and Infectious Diseases [Bethesda] (NIAID-NIH), National Institutes of Health [Bethesda] (NIH), Nanyang Technological University [Singapour], Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Microbiology, National University of Singapore (NUS)-Yong Loo Lin School of Medicine, Institut de Recherches Internationales Servier [Suresnes] (IRIS), This research was funded by government funding to SIgN, A*STAR, Singapore, and supported by the NIH/NIAID., Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Singapore Immunology Network, BMSI, A-STAR, National Institute of Allergy and Infectious Diseases [Bethesda], National Institutes of Health ( NIH ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), National University of Singapore ( NUS ) -Yong Loo Lin School of Medicine, Institut de Recherches Internationales Servier [Suresnes] ( IRIS ), School of Biological Sciences, Bos, Mireille
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
Cell Survival
Population
Tumor-initiating cell
Metastasis
Mice
03 medical and health sciences
TGFβ
0302 clinical medicine
Transforming Growth Factor beta
mental disorders
medicine
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
General hospital
education
Melanoma
[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry
Molecular Biology
Cell Proliferation
030304 developmental biology
0303 health sciences
education.field_of_study
biology
Arginase
Macrophages
Transforming growth factor beta
M2 Macrophage
medicine.disease
Mice
Mutant Strains
3. Good health
Science::Biological sciences [DRNTU]
Disease Models
Animal
Oncology
030220 oncology & carcinogenesis
Immunology
Neoplastic Stem Cells
Cancer research
biology.protein
Female
Signal Transduction
Research Paper
Transforming growth factor
Zdroj: Oncotarget
Oncotarget, Impact journals, 2014, pp.25294815
Oncotarget, 2014, pp.25294815
ISSN: 1949-2553
Popis: // Muly Tham 1 , Kar Wai Tan 1,7 , Jo Keeble 1 , Xiaojie Wang 1 , Sandra Hubert 1 , Luke Barron 2 , Nguan Soon Tan 3 , Masashi Kato 4 , Armelle Prevost-Blondel 5 , Veronique Angeli 6 and Jean-Pierre Abastado 1,8 1 Singapore Immunology Network, BMSI, A-STAR, Singapore 2 Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA 3 School of Biological Sciences, Nanyang Technological University, Singapore 4 Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Japan 5 Institut Cochin, Universite Paris Descartes, CNRS UMR, Paris, France 6 Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 7 Department of Clinical Research, Singapore General Hospital, Singapore 8 Institut de Recherche Internationales Servier, 50 rue Carnot, Suresnes cedex, France Correspondence: Muly Tham, email: // Keywords : Arginase, Macrophages, TGFβ, Tumor-initiating cell Received : July 13, 2014 Accepted : September 15, 2014 Published : September 16, 2014 Abstract M2 macrophages promote tumor growth and metastasis, but their interactions with specific tumor cell populations are poorly characterized. Using a mouse model of spontaneous melanoma, we showed that CD34 - but not CD34 + tumor-initiating cells (TICs) depend on M2 macrophages for survival and proliferation. Tumor-associated macrophages (TAMs) and macrophage-conditioned media protected CD34 - TICs from chemotherapy in vitro . In vivo , while inhibition of CD115 suppressed the macrophage-dependent CD34 - TIC population, chemotherapy accelerated its development. The ability of TICs to respond to TAMs was acquired during melanoma progression and immediately preceded a surge in metastatic outgrowth. TAM-derived transforming growth factor-β (TGFβ) and polyamines produced via the Arginase pathway were critical for stimulation of TICs and synergized to promote their growth.
Databáze: OpenAIRE
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