Infection and Inflammation in Skeletal Muscle fromNonhuman Primates Infected with Different Genospecies of theLyme Disease SpirocheteBorreliaburgdorferi
| Autor: | Kavi Narayan, Emir Hodzic, Stephen W. Barthold, Diego Cadavid, Yunhong Bai, Andrew R. Pachner, Donna Dail, Marie Hurd |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Immunology Anti-Inflammatory Agents Spirochaetaceae Microbiology Dexamethasone Lyme disease Image Processing Computer-Assisted otorhinolaryngologic diseases medicine Animals Humans Bites and Stings Borrelia burgdorferi Muscle Skeletal Myositis Immunosuppression Therapy Inflammation Lyme Disease Ixodes biology Bacterial Infections bacterial infections and mycoses biology.organism_classification medicine.disease Antibodies Bacterial Immunohistochemistry Macaca mulatta Virology Disease Models Animal Infectious Diseases Needles biology.protein Lyme disease microbiology Parasitology Immunocompetence Antibody |
| Zdroj: | Infection and Immunity. 71:7087-7098 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.71.12.7087-7098.2003 |
| Popis: | Lyme borreliosis is a multisystemic disease caused by various genospecies of the spirocheteBorrelia burgdorferi. To investigate muscle involvement in the nonhuman primate (NHP) model of Lyme disease, 16 adultMacaca mulattaanimals inoculated with strain N40 ofB. burgdorferisensu strictu by syringe or by tick bite or with strain Pbi ofB. burgdorferigenospeciesgariniiby syringe were studied. Animals were necropsied while immunosuppressed on day 50 (two animals each inoculated withB.burgdorferiN40 by syringe and withB.gariniiPbi by syringe) or on day 90, 40 days after immunosuppression had been discontinued (four animals each inoculated with strain N40 by syringe, with strain N40 by tick bite, and with strain Pbi by syringe). Skeletal muscles removed at necropsy were studied by (i) microscopic examination of hematoxylin-eosin-stained sections for inflammation and tissue injury; (ii) immunohistochemical and digital image analyses for antibody and complement deposition and cellular inflammation; (iii) Western blot densitometry for the presence of antibodies; and (iv) reverse transcription-PCR for measurement of the spirochetal load or C1q (the first component of the complement cascade) synthesis. The results showed that N40 was more infectious for NHPs than Pbi. NHPs inoculated with N40 but not with Pbi developed myositis. The inflammation in skeletal muscle was more severe in NHPs inoculated with N40 by syringe than in those inoculated by tick bite. The predominant cells in the inflammatory infiltrate were T cells and plasma cells. The deposition of antibody and complement in inflamed muscles from N40-inoculated NHPs was significantly higher than that in Pbi-inoculated NHPs. The spirochetal load was very high in the two N40-inoculated NHPs examined while they were immunosuppressed but decreased to minimal levels in the NHPs when immunocompetence was restored. We conclude that myositis can be a prominent feature of Lyme borreliosis depending on the infecting organism and host immune status. |
| Databáze: | OpenAIRE |
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