Field Evaluation of the Performance of Two Rapid Diagnostic Tests for Meningitis in Niger and Burkina Faso
| Autor: | Chantal Kambire, Haladou Moussa, Halima Boubacar Maïnassara, Muhamed-Kheir Taha, Katya Fernandez, Fati Sidikou, Mamadou Tamboura, Olivier Ronveaux, Kambire Dinanibe, Marc Rondy, Baruani Bienvenu, Guetwende Sawadogo, Issaka Yaméogo, Rasmata Ouédraogo, Ali Elhaj Mahamane |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty Concordance 030106 microbiology Neisseria meningitidis rapid diagnostic test medicine.disease_cause Microbiology Article cerebrospinal fluid 03 medical and health sciences 0302 clinical medicine Virology Positive predicative value Internal medicine Burkina Faso Streptococcus pneumoniae Medicine Niger 030212 general & internal medicine lcsh:QH301-705.5 Rapid diagnostic test business.industry meningitis Outbreak Diagnostic test medicine.disease lcsh:Biology (General) national reference laboratory business Meningitis |
| Zdroj: | Microorganisms, Vol 9, Iss 832, p 832 (2021) Microorganisms Volume 9 Issue 4 |
| ISSN: | 2076-2607 |
| Popis: | New lateral flow tests for the diagnosis of Neisseria meningitidis (Nm) (serogroups A, C, W, X, and Y), MeningoSpeed, and Streptococcus pneumoniae (Sp), PneumoSpeed, developed to support rapid outbreak detection in Africa, have shown good performance under laboratory conditions. We conducted an independent evaluation of both tests under field conditions in Burkina Faso and Niger, in 2018–2019. The tests were performed in the cerebrospinal fluid of suspected meningitis cases from health centers in alert districts and compared to reverse transcription polymerase chain reaction tests performed at national reference laboratories (NRLs). Health staff were interviewed about feasibility. A total of 327 cases were tested at the NRLs, with 26% confirmed Nm (NmC 63% and NmX 37%) and 8% Sp. Sensitivity and specificity were, respectively, 95% (95% CI: 89–99) and 90% (95% CI: 86–94) for Nm and 92% (95% CI: 75–99) and 99% (95% CI: 97–100) for Sp. Positive and negative predictive values were, respectively, 77% (95% CI: 68–85) and 98% (95% CI: 95–100) for Nm and 86% (95% CI: 67–96) and 99% (95% CI: 98–100) for Sp. Concordance showed 82% agreement for Nm and 97% for Sp. Interviewed staff evaluated the tests as easy to use and to interpret and were confident in their readings. Results suggest overall good performance of both tests and potential usefulness in meningitis outbreak detection. |
| Databáze: | OpenAIRE |
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