Lipid composition in a strain of Bacillus subtilis, a producer of iturin A lipopeptides that are active against uropathogenic bacteria
| Autor: | Grażyna Płaza, Katarzyna Paraszkiewicz, Przemysław Bernat, Magdalena Moryl, Paulina Siewiera, Joanna Chojniak |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Catheters Physiology 030106 microbiology Microbial Sensitivity Tests Bacillus subtilis medicine.disease_cause Peptides Cyclic Applied Microbiology and Biotechnology Enterococcus faecalis Microbiology Lipopeptides 03 medical and health sciences chemistry.chemical_compound Iturin Tandem Mass Spectrometry medicine Humans Uropathogens Escherichia coli Phospholipids Original Paper Bacteria biology Fatty Acids General Medicine biology.organism_classification Proteus mirabilis Anti-Bacterial Agents Citrobacter freundii 030104 developmental biology chemistry Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Lipidomics Serratia marcescens lipids (amino acids peptides and proteins) Urinary Catheterization Surfactin Chromatography Liquid Biotechnology |
| Zdroj: | World Journal of Microbiology & Biotechnology |
| ISSN: | 0959-3993 |
| DOI: | 10.1007/s11274-016-2126-0 |
| Popis: | Urinary tract infections are a common disease in humans. Therefore, new methods are needed to destroy biofilms that are formed by uropathogens. Iturin A lipopeptides (LPs) C14 and C15 are potent biosurfactants synthetized by the Bacillus subtilis I′1a strain. The biological activity of extracted LPs was confirmed by examining extracts from I′1a cultures against uropathogenic bacteria that had been isolated from biofilms on urinary catheters. Compared with cultures of DSM 3257, which produce surfactin at a relatively low level, the extract obtained from strain I′1a exhibited a greater inhibitory effect against both planktonic and sessile forms of Escherichia coli, Serratia marcescens, Enterobacter cloacae, Proteus mirabilis, Citrobacter freundii and Enterococcus faecalis. Moreover, cyclic LP biosurfactants may disturb the integrity of cytoplasmic membranes; therefore, we investigated the effects of synthetized LPs on fatty acids and phospholipids of B. subtilis. LPs and lipids were analyzed using GC–MS, LC–MS/MS and MALDI-TOF/TOF techniques. Compared with B. subtilis DSM 3257, membranes of the I′1a strain were characterized by an increased amount of anteiso fatty acids and a ten-fold higher ratio of phosphatidylglycerol (PG)-to-phosphatidylethanolamine (PE). Interestingly, in cultures of B. subtilis DSM 3257 supplemented with LP extracts of the I′1a strain, the PG-to-PE ratio was fourfold higher, and the amount of anteiso fatty acids was also increased. Graphical Abstract |
| Databáze: | OpenAIRE |
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