A molecularly imprinted receptor for separation of testosterone and epitestosterone, based on a steroidal cross-linker
| Autor: | Alan D. Brailsford, Karen Mitchell, Richard J. Ansell, Ivana Gavrilović, Andrew T. Kicman, David A. Cowan |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Polymers
medicine.medical_treatment Clinical Biochemistry Biochemistry High-performance liquid chromatography Steroid Molecular Imprinting chemistry.chemical_compound Endocrinology Molecular recognition Methods medicine Testosterone Molecular Biology Chromatography High Pressure Liquid Pharmacology chemistry.chemical_classification Chromatography Organic Chemistry Epitestosterone Molecularly imprinted polymer Polymer Divinylbenzene Cross-Linking Reagents chemistry Steroids Molecular imprinting medicine.drug |
| Zdroj: | Steroids. 76:478-483 |
| ISSN: | 0039-128X |
| Popis: | A series of molecularly imprinted polymers have been prepared and investigated as stationary phases in high performance liquid chromatography for the separation of testosterone and epitestosterone using non-polar mobile phases. The polymers were imprinted using 5α-dihydrotestosterone as template, and all retain testosterone more strongly than its 17α-OH epimer. The best polymer was prepared using trifluoromethylacrylic acid as functional monomer (interacting with the template via hydrogen bonds), divinylbenzene as ‘inert’ cross-linker, and chloroform as porogen. It also included a steroid-based cross-linker, which may interact with the template via van der Waals interactions to lend additional ‘shape selectivity’. A 250 × 4.6 mm column packed with this polymer gave baseline resolution of testosterone and epitestosterone (15 μg each) in under 20 min. Preparation of the steroid based cross-linker included the selective reduction of 5α-dihydrotestosterone (17β-hydroxy-5α-androstan-3-one) to the 3α,17β-diol using K-selectride. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect