Analysis of bacterial communities on historical glass by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA
| Autor: | Werner Lubitz, Rudolf Weissmann, Ursula Drewello, Rainer Drewello, Claudia Gurtner, Sabine Rölleke |
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| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Microorganism Molecular Sequence Data Biology Microbiology DNA Ribosomal Polymerase Chain Reaction law.invention law RNA Ribosomal 16S Molecular Biology Ribosomal DNA Polymerase chain reaction Ecosystem Gel electrophoresis Electrophoresis Agar Gel Bacteria Gene Amplification Genes rRNA Flat glass 16S ribosomal RNA biology.organism_classification Glass Temperature gradient gel electrophoresis |
| Zdroj: | Journal of microbiological methods. 36(1-2) |
| ISSN: | 0167-7012 |
| Popis: | The present study describes the analysis of bacterial communities on historical window glass by denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA fragments. So far, only a few studies have been published in which the microflora and the corrosion mechanisms of glass surfaces have been investigated. Some microorganisms, especially fungi, have been isolated from different glass samples in the past. However, our results demonstrate that bacterial communities on biodeteriorated glass surfaces are much more complex than previously believed. In addition, bacteria were identified, which have never been isolated from glass samples before. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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